Sequences from confirmation amplicon


Confirmation sequences for line 915C02 and hit At2g22155 are shown.
Line ID 915C02
Locus At2g22155 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9424105
Primers used for PCR 17nw - 8474, show primer details
   
Sequence Name 55-K114664-0022-915-C02C-17nw
GenBank Accession LN508888 [GenBank]
Sequencing primer gene specific primer, 17nw: 5'-AAATTTACAAAAACGTTCAGGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114664-0022-915-C02C-17nw
TTATTCTTTGGTATATATATATGATACGTAAATGTATTTTGTAGTGAGTGTACCTATATA
TACACGTACACAAAATTAAGTGACATTGTTGAAAACGCTATCTTTTATAAAGGAAATGAT
CATGAATTTGAATGGCATATATACCAAGATATATGGAACTCTATTATCATATCTGCTAGG
TGAAATGAGCAAACGAAAATCTACCAATTTGAATAAACTCATATCTCTAGTCATTCTACC
AAAACAAAGTATAAAAAGCTCCACCATCATTAACATATTACAAAAAAAAAACTAAACAAT
TTTTTGGGTTTTTTAATTTAATTATAGAAAAAATGGAATCTAAGACAATGAAAATTATAA
TTTTTCTTAGGGTTTTTGTTTTTGCAATGACTTGGTCTCCTTCAAGTGCTCAACCAACGG
ATCCGGAGGGGTGTTTTAAGGCGTTTAAAAGCACATCAAGAGGATGTCTCGAAAGCATAA
AAGGAATTTTACATGGACATGTCCATGGGATCAAAAAAGAATGTTGTGAAACCGTTAGTA
TAGTTTCTGATATGTGTTGGCCAATAATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAAATGTCC
   
Sequence Name 26-K114663-0022-915-C02C-8409
GenBank Accession LN498041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114663-0022-915-C02C-8409
TTGAATTATTGGCCAACCATATCAGAAACTATACTAACGGCTTCTCAACATTCTTTCTTG
ATCCCATGGACATGTCCATGTCATATTCCTTTTATGCTTTCGAGACATCCTCTTGATGTG
CTTTTAAACGCCTTAAAACACCCCTCCGGATCCGTTGGTTGAGCACTCGAAGGAGACCAA
GTCATTGCAAAAACAAAAACCATAAGAAAAATTATAATCTTCATCGTCTTAGATTCCATT
TTTTCTATAATTAAATTAAAAAACCCAAAAAATTGTTTAGTTTTTTTTTTGTAATATGTT
AATGATGGTGGAGCTTTTTATACTTTGTTTTGGTAGAATGACTAGAGATATGAGTTTATT
CAAATTGGTAAATTTTCGTTTGCTCATTTCACCTAGCAAATATGATAATAGAGTTCCATA
TATCTGGGTATATATGCCATTCAAATTCATGATCATTTCCTTTATAAAAGATAGCCTTTT
CAACAATGTCACTTAATTTTGTGTACGTGGACTTATAATACACTCACTACAAAATACATT
TACGTATCATATATATTTTACCAAAGTAATTAAGCATTTCGCCAACTAGAGAGTGCCACC
TGAAC

Last Updated on 06.12.2018 16:11