Sequences from confirmation amplicon


Confirmation sequences for line 915F02 and hit At3g01700 are shown.
Line ID 915F02
Locus At3g01700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:259513
Primers used for PCR 19nw - 8474, show primer details
   
Sequence Name 68-K114716-0022-915-F02C-19nw
GenBank Accession LN512067 [GenBank]
Sequencing primer gene specific primer, 19nw: 5'-ACACAAAATAAGAAAGAAAGGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K114716-0022-915-F02C-19nw
CGAGAATATACCACTAGACCAAATGCCCTCTTTGAAAAATTTGTTTTTCATTTAATTTCT
AAATAAACATTAATAAGTTCACAATCATCAAAAATTACGTAATCAAAAAAAACATAGTAC
AACAACGGTACGTAATAATAATAACTCCAAATGATATAACAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAGTAGATGTCCGCGCGTTATAATAA
   
Sequence Name 20-K114715-0022-915-F02C-8409
GenBank Accession LN495322 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114715-0022-915-F02C-8409
ATTTACAATTGTTATATCATTTGGAGTTATTATTATTACGTACCGTTGTTGTACTATGTT
TTTTTTGATTACGTAATTTTTGATGATTGTGAACTTATTAATGTTTATTTAGAAATTAAA
TGAAAAACAAATTTTTCAAAGAGGGCATTTGGTCTAGTGGTATGATTCTCGCTTAGGGTG
CGAGAGGTCCCGAGTTCAATTCTCGGAATGCCCCCTTTCTTTCTTATTTTGTG

Last Updated on 06.12.2018 16:11