Sequences from confirmation amplicon


Confirmation sequences for line 917B02 and hit At1g77370 are shown.
Line ID 917B02
Locus At1g77370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29074569
Primers used for PCR 49lm - 8474, show primer details
   
Sequence Name 41-K114022-0022-917-B02C-49lm
GenBank Accession LN504157 [GenBank]
Sequencing primer gene specific primer, 49lm: 5'-GTCATCTTCTCCAAATCCTACTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114022-0022-917-B02C-49lm
TTTGAATTTGCATTTAGGAACTTTCCAAGTTTTGGTTCATATCTAGCTTTCCTTATTCAT
TGGCACGATGGCGGAATTCGAAATTCCAAGACTTTGTGTTGAAAGTTGTGAATTTTGTTT
TGGATGTGTTAGGTATTGTTTGCGGTCGAAACGTATATTCAGCCAACTTAAGGAAGAGCC
ATTTGTTGTGGAGCTTGATCAGAGAGGTATCACTTTCTTTCTCACCCAACTGTTTATGAG
AATTGAACTGTTTCTTAAAACTTCTGAGAGATTTGTTTTTGCAGAGGACGGAGATCAAAT
CCAGTATGAGCTTTTAGAATTCGTTGGTCGTCGTACTGTCCCGCAAGTTTTTGTTAACGG
CAAGCATATTGGTGGATCAGATGGTATGATGAGCTCACTATCTCCTCCAAGTTTCATTCT
TTTGTCCAAATTTCCCTTGTAACTCACAAGCCTTGGCTGTTTATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 20-K114021-0022-917-B02C-8409
GenBank Accession LN495299 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114021-0022-917-B02C-8409
ACAGCCAAGGCTTGTGAGTTCAAGGGAAATTTGGACAAAAGAATGAAACTTGGAGGAGAT
AGTGAGCTCATCATACCATCTGATCCACCAATATGCTTGCCGTTAACAAAAACTTGCGGG
ACAGTACGACGACCAACGAATTCTAAAAGCTCATACTGGATTTGATCTCCGTCCTCTGCA
AAAACAAATCTCTCAGAAGTTTTAAGAAACAGTTCAATTCTCATAAACAGTTGGGTGAGA
AAGAAAGTGATACCTCTCTGATCAAGCTCCACAACAAATGGCTCTTCCTTAAGTTGGCTG
AATATACGTTTCGACCGCAAACAATACCTAACACATCCAAAACAAAATTCACAACTTTCA
ACACAAAGTCTTGGAATTTCGAATTCCGCCATCGTGCCAATGAATAAGGAAAGCTAGATA
TGAACCAAAACTTGGAAAGTTGCCTAAATTGCAAATTTCAAATTAGGAGAAAGAGTGAAA
TTTAATTACGGGCAGTAGGATTTGAGAAAAGATGA

Last Updated on 06.12.2018 16:11