Sequences from confirmation amplicon


Confirmation sequences for line 917B04 and hit At1g72760 are shown.
Line ID 917B04
Locus At1g72760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27386132
Primers used for PCR 65by - 8474, show primer details
   
Sequence Name 87-K108479-0022-917-B04C-65by
GenBank Accession LN514774 [GenBank]
Sequencing primer gene specific primer, 65by: 5'-TGTTCACAACGGTTTCTTACAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K108479-0022-917-B04C-65by
GCACCTATGGTTTTTCTGATGCTCTAAGATTGGGGAAGGAGGTTATGGACCTGTTTACAA
AGCTGTCCTCGATTACACTTCTGTCGCCATTAAAATCTTGAAGTCGGGTATCACAGAGGG
ACTGAAACAGTTCCAACAAGAGGTACTGTGTTAGTTTATATTGCTCTCCTTATTTTGCTT
CTCCTTCTCCTGACTCATACATATGTTTCTTCAATAGATTGAGGTTCTTAGTAGCATGAG
GCACCCTAATATGGTAATCCTTCTCGGTGCATGTCCCGAGTATGGTTGTCTTGTATATGA
GTATATGGAAAATGGAACACTCGAAGATCGCCTCTTCTGCAAAAACAACACACCCCCACT
TTCATGGAGAGCAAGGTTCAGAATCGCTTCTGAGATTGCCACAGGTCTTCTTTTCCTTCA
CCAGGCAAAACCAGCAATAGGCGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 75-K108478-0022-917-B04C-8409
GenBank Accession LN513255 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K108478-0022-917-B04C-8409
AAGAGACCTGTGGCAATCTCAGAAGCGATTCTGAACCTTGCTCTCCATGAAAGTGGGGGT
GTGTTGTTTTTGCAGAAGAGGCGATCTTCGAGTGTTCCATTTTCCATATACTCATATACA
AGACAACCATACTCGGGACATGCACCGAGAAGGATTACCATATTAGGGTGCCTCATGCTA
CTAAGAACCTCAATCTATTGAAGAAACATATGTATGAGTCAGGAGAAGGAGAAGCAAAAT
AAGGAGAGCAATATAAACTAACACAGTACCTCTTGTTGGAACTGTTTCAGTCCCTCTGTG
ATACCCGACTTCAAGATTTTAATGGCGACAGAAGTGTAATCGAGGACAGCTTTGTAAACA
GGTCCATAACCTCCTTCCCCAATCTTTAGAGCATCAGAAAAACCATAGGTTGCATCTTCA
ACATCTTTGATACTATAACGTCTGTAAGAAACCGTTGTGAAC

Last Updated on 06.12.2018 16:11