Sequences from confirmation amplicon


Confirmation sequences for line 918E10 and hit At4g16730 are shown.
Line ID 918E10
Locus At4g16730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9404549
Primers used for PCR 42wx - 8474, show primer details
   
Sequence Name 60-K119070-0022-918-E10C-42wx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 42wx: 5'-GTCCAGTTTTGTACCACTTGGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K119070-0022-918-E10C-42wx
TAAATTAAAAATTTCTAAGTAACATCATTTTCTATATACTAAAGGATTTGTAGAAAAACT
TACAACTTGTTTGAGGTAAGGGATAACGTTAAGTCCTTTGTCTCTGAGAACATCGTATCC
TATTTGGTTGATCTCATTGTATAGAAAAAGAAAACACATTCTCATGTAGTTGGGAAGTCT
TTCAACATGGTTCACATCCCATCTGGAAAAGAGTATATTATATGGATTATAATTAACTGA
TATTATGACTGTTGAAAAAAATAAAAATTATCATGGGAGACACAATTCAGAATATGATCT
TTTTATACCAAAAATCATCTTAAAAAGTTTTCAAACATATTGTTATTTAAAAGTTTCTTA
TACTTATACGATATACTTACTTTTCGACCACATTTGTGAAAAGCTCGAGCTCCTCTAATG
TACCATAGATGTCGTAGATATCATCCATAATCACAATTAACATGAATATTTTGGTGAGTA
TCTTGCGGCAGTATTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGGTTATT
   
Sequence Name 28-K119069-0022-918-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K119069-0022-918-E10C-8409
CATTTAATCTGCCGCAAGATCTCACCAAAATATTCATGTTAATTGTGCTTATGGATGATA
TCTACGACATCTATGGTACATTAGAGGAGCTCGAGCTTTTCACAAATGTGGTCGAAAAGT
AAGTATATCGTATAAGTATAAGAAACTTTTAAATAACAATATGTTTGAAAACTTTTTAAG
ATGATTTTTGGTATAAAAAGATCATATTCTGAATTGTGTCTCCCATGATAATTTTTATTT
TTTTCAACAGTCATAATATCAGTTAATTATAATCCATATAATATACTCTTTTCCAGATGG
GATGTGAACCATGTTGAAAGACTTCCCAACTACATGAGAATGTGTTTTCTTTTTCTATAC
AATGAGATCAACCAAATAGGATACGATGTTCTCAGAGACAAAGGACTTAACGTTATCCCT
TACCTCAAACAAGTTGTAAGTTTTTCTACAAATCCTTTAGTATATAGAAAATGATGTTAC
TTAGAAATTTTTAATTTTATTTACGTAGTGGACGGATCTATTTAAAACGTTCCTAACTGA
ATCCAAGTGGTACAAAACTGGA

Last Updated on 06.12.2018 16:11