Sequences from confirmation amplicon


Confirmation sequences for line 920G09 and hit At5g15810 are shown.
Line ID 920G09
Locus At5g15810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5160958
Primers used for PCR 42lx - 8474, show primer details
   
Sequence Name 81-K114129-0022-920-G09C-42lx
GenBank Accession LN514097 [GenBank]
Sequencing primer gene specific primer, 42lx: 5'-CCATTTAAGTCTAAAGGCACAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K114129-0022-920-G09C-42lx
TGAATGAACTCGTTTGTTTGATTGTAGGTGAAAAATCATCCCATAAAGCCGCAATCACCT
GAACATCCTGGAAGTGTGATTCTATCTAAAGAACCATCTCATCAGGTCTCTCTTTCACTA
AGCCATTGTTGTAATGCTTCATTCATTGTTTATATATTTAAAGTTCCAAACTTGAATCTT
TAAACACCGCTTTCTTTCGGACAGGCTGACTTTTCGCGCCACGTCGGTTCGCTTAGTAAA
GCACAGGCGAAGAAAGTAGCCCGGTTTCTACCAAACCCAGAGAAGCATTGGGGTCCCAAG
ATAAGGGCAGGTCGTACAATCACAAGCAAACATGTATCGCTTCTTGGTCATGAAGCAATA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGACCGCACGGTTATT
   
Sequence Name 39-K114128-0022-920-G09C-8409
GenBank Accession LN503432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114128-0022-920-G09C-8409
CAGAGCGATACATGTTTGCTTGTGATTGTACGACCTGCCCTTATCTTGGGACCCCAATGC
TTCTCTGGGTTTGGTAGAAACCGGGCTACTTTCTTCGCCTGTGCTTTACTAAGCGAACCG
ACGTGGCGCGAAAAGTCAGCCTGTCCGAAAGAAAGCGGTGTTTAAAGATTCAAGTTTGGA
ACTTTAAATATATAAACAATGAATGAAGCATTACAACAATGGCTTAGTGAAAGAGAGACC
TGATGAGATGGTTCTTTAGATAGAATCACACTTCCAGGATGTTCAGGTGATTGCGGCTTT
ATGGGATGATTTTTCACCTACAATCAAACAAACGAGTTCATTCATTATCAATGAGGAAAA
GAAATGACATTCAAAGCTTGTGCCTTTAACNTTAAATGG

Last Updated on 06.12.2018 16:11