Sequences from confirmation amplicon


Confirmation sequences for line 921D08 and hit At1g22490 are shown.
Line ID 921D08
Locus At1g22490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7939535
Primers used for PCR 22um - 8474, show primer details
   
Sequence Name 36-K117387-0022-921-D08C-22um
GenBank Accession LN502383 [GenBank]
Sequencing primer gene specific primer, 22um: 5'-TATCATTTGGTCAGGGTCATTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117387-0022-921-D08C-22um
TCATTTCTCCATAATATATTAACACAAAAGAATAGAATGAAAATGACTGAGAATATATAT
TGATGTTTTTATACAATATATTAACAAGAAAATCCACCGTAAATATGGAAGGGTGACGCG
TTTATATACATACTAATAATCTTTGCTGTTACATATTTTAATGTATTTTTTAAAATAAAA
TATTATGTGTCACCAAAAATTAGAACGAGTATGATTGAGAATATTGGGATATGTTTTAGA
ACAATTTCGTAATAAACTTAAGCAATTAGCAATGATACGTGTGTGTGTATATTTATACAT
TCAAAGATTTTTTCAATCTTTATGATAACTATATGATCTGACATGAGCGGGTTTTGGTGG
TAAAGGGAGATCAAGCGTCGATAGTAGGAGGAGCTATAAACTACGTGAAGGAGTTACCAA
TTGGTGTCACGCTCCCAATGGCTTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K117386-0022-921-D08C-8409
GenBank Accession LN490372 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117386-0022-921-D08C-8409
ATTTCATTGAATATGAAGCCATTGGGAGCGTGACACCAATTGGTAACTCCTTCACGTAGT
TTATAGCTCCTCCTACTATCGACGCTTGATCTCCCTTTACCACCAAAACCCGCTCATGTC
AGATCATATAGTTATCATAAAGATTGAAAAAATCTTTGAATGTATAAATATACACACACA
CGTATCATTGCTAATTGCTTAAGTTTATTACGAAATTGTTCTAAAACATATCCCAATATT
CTCAATCATACTCGTTCTAATTTTTGGTGACACATAATATTTTATTTTAAAAAATACATT
AAAATATGTAACAGCAAAGATTATTAGTATGTATATAAACGCGTCACCCTTCCATATTTA
CGGTGGATTTTCTTGTTAATATATTGTATAAAAACATCAATATATATTCTCAGTCATTTT
CATTCTATTCTTTTGTGTTAATATATTATGGAGAAATGAGTACTACAGCCAAGCAAGTAA
TAAAACAAAGACACATATACCTAAGAATGACCCTGACAAAATGAT

Last Updated on 06.12.2018 16:11