Sequences from confirmation amplicon


Confirmation sequences for line 922H06 and hit At5g56020 are shown.
Line ID 922H06
Locus At5g56020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22684686
Primers used for PCR 27fx - 8474, show primer details
   
Sequence Name 79-K111632-0022-922-H06C-27fx
GenBank Accession LN513843 [GenBank]
Sequencing primer gene specific primer, 27fx: 5'-ACCACTTGCTAGAAGTAAACCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K111632-0022-922-H06C-27fx
TACTTGTTGCAGACTGCAGGTTATCTCTAACTCCTTTCGAAACACTGTTAACAATCCAGA
TTTGAGTACAACAAAATTCAAAATCAAAAAGTTAGTAACACTTCAAGAAAAAGATATGCT
TAAGACTCTTAATCAATTCTAAGTCATTGAGAGAAGACACTAATCAAAATCATTCACTAA
CAATAGCTTCAAACCCTGAGAATTTGTAAATCTGATCAACGAATCTGGCAAAGCTTAAAC
TATATGGTTAATCACAATTTCTCAGATTCCAGATTTAATTTACACCAATCCAATCCTCAA
TAGCAGGCGAAAACGCCTAATTTCTCAGATACGGTCACGATCAATCGTTAAACCCCTAAA
GATTAAGAGAAAGAGATGGATGGATTACACATTAAAGGTGCCGGAAACAGTGTCGTTGGC
AGATCTAACGGCGGATTCGATATCGAAGCCTAAAGTTCCGGTGTCTTCTTCAAAATCTCT
CGATGCTGCGTAAGAATTCCAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT
   
Sequence Name 63-K111631-0022-922-H06C-8409
GenBank Accession LN511018 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K111631-0022-922-H06C-8409
ATTTACATTGAATATATCCTGATTGGAATTCTTACGCAGCATCGAGAGATTTTGAAGAAG
ACACCGGAACTTTAGGCTTCGATATCGAATCCGCCGTTAGATCTGCCAACGACACTGTTT
CCGGCACCTTTAATGTGTAATCCATCCATCTCTTTCTCTTAATCTTTAGGGGTTTAACGA
TTGATCGTGACCGTATCTGAGAAATTAGGCGTTTTCGCCTGCTATTGAGGATTGGATTGG
TGTAAATTAAATCTGGAATCTGAGAAATTGTGATTAACCATATAGTTTAAGCTTTGCCAG
ATTCGTTGATCAGATTTACAAATTCTCAGGGTTTGAAGCTATTGTTAGTGAATGATTTTG
ATTAGTGTCTTCTCTCAATGACTTAGAATTGATTAAGAGTCTTAAGCATATCTTTTTCTT
GAAGTGTTACTAACTTTTTGATTTTGAATTTTGTTGTACTCAAATCTGGATTGTTAACAG
TGTTTCGAAAGGAGTTAGAGATAACCTGCAGTCTGCAACAAGTAGTATGCCTTCAGGAAA
AGCTTTGATGTATTTCGGTTTACTTCTGCCAAGTGG

Last Updated on 06.12.2018 16:11