Sequences from confirmation amplicon


Confirmation sequences for line 923D03 and hit At3g23990 are shown.
Line ID 923D03
Locus At3g23990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8670551
Primers used for PCR 22rx - 8474, show primer details
   
Sequence Name 20-K117167-0022-923-D03C-22rx
GenBank Accession LN495386 [GenBank]
Sequencing primer gene specific primer, 22rx: 5'-AGGATGGAAAGACATTGTTTAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117167-0022-923-D03C-22rx
TTCACCATACTTTATCACTAATCAAAAAACCCAAAAATGTGTAAGTTTTTTACTCCCTGC
TCAATTAAGTTTCTTGATGTGTCATATCAATTATAGAAGTTACTGAATTGGAATTAATCT
ATGTAGGAACTAGACGATCCTCTCATTCTTATCCACGAGAAGAAGATCTCAAGCATCAAC
TCTATAGTGAAAGTGTTGGAGTTGGCTCTGAAGGTATGTCTACTTTATCTTTATAGTAAA
TCTGTAATCATTTTCTCATGTAAATGCTGTGTGAAAGGCATCTGATGTGGTTTCTGTTGT
CCAAATAAAATCTTACAGAGGCAAAGACCACTATTGATTGTTGTCCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 09-K117166-0022-923-D03C-8409
GenBank Accession LN490369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117166-0022-923-D03C-8409
GCATTTCATTGAATATATCCTGGACAACATCAATAGTGGTCTTTGCCTCTGTAAGATTTT
ATTTGGACAACAGAAACCACATCAGATGCCTTTCACACAGCATTTACATGAGAAAATGAT
TACAGATTTACTATAAAGATAAAGTAGACATACCTTCAGAGCCAACTCCAACACTTTCAC
TATAGAGTTGATGCTTGAGATCTTCTTCTCGTGGATAAGAATGAGAGGATCGTCTAGTTC
CTACATAGATTAATTCCAATTCAGTAACTTCTATAATTGATATGACACATCAAGAAACTT
AATTGAGCAGGGAGTAAAAAACTTACACATTTTTGGGTTTTTTGATTAGTGATAAAGTAT
GGTGAAGTATAACCTCTGTCCAATTTCATCCCCTCTACAACTTCCAACTCGTTAAACAAT
GTCTTTCCATCCT

Last Updated on 06.12.2018 16:11