Sequences from confirmation amplicon


Confirmation sequences for line 923G03 and hit At4g03330 are shown.
Line ID 923G03
Locus At4g03330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1467180
Primers used for PCR NR78 - 8474, show primer details
   
Sequence Name 34-K102514-0022-923-G03C-8409
GenBank Accession LN501368 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K102514-0022-923-G03C-8409
ATTTACAATTTTTGAATAAGATTTGATCATTGTTCAATCATAGTAAAAGGATTTGGTCTT
TGTTGGATCAGAGTAACACCTAAATGAAAGAAAAAGAAATAAAATTTACCTTCTCTCGAC
CGTTTCTTTGTATTCAGTAGCCATTTTGGTTCGTAGTCTCTGAAAATCATCCATCATGTC
TTTAAGCTTCTTCCCCAATCCACTAACGACCGATGTTCTGGTCCGATCAGCAGACGAGCC
AGGACCACAACCAGCGACTTTCCTCTGCGCAGCATTTGATTTCTCCAAAGCTACAAGCTT
AGTCTTTATCATCTTGACACGTTTCAAGACCTCTGTAACACTCGAATCCATACGAGCACG
AAGCTTCTTCACGGCCTTGGAATCATGTACGGTCTTGGACTCTTCATTAGCATCTTGGAG
ACGCTTATGAATCTCGTCGACAGCTTTCATGTCTTCTTTAACACTCTCGACATATCCAAA
GAACTCATCGAGGTTGCCTGAATCAAGAGAAACATTTTGAGACTCGATGTCATCGAGTTG
GACCTGGTGGTTTAAGTCTGTGTATCTCTTGAATGAGCTTGAGATAAGATCGTTCATGGC
GACGATCAAGAACTTAGAGAAAAATGATTTCTAATTTCTTGAAAATGGGCAGAGGAACAT
TAATGGTGGTTGGTTTAGATGGTTGACATGAAGCTAGAGAGACCTTTAAGAGCCGTCTGA
AACCAATGTGTAAAATACTAAATAGACATATAATGAGCTGTGTTTAGAACCAGTCAAGAG
TTTCAGACC
   
Sequence Name 53-K102515-0022-923-G03C-NR78
GenBank Accession LN508113 [GenBank]
Sequencing primer gene specific primer, NR78: 5'-CAAAGTCTTCCTTGGTCTTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K102515-0022-923-G03C-NR78
GCTCATTAATGTCTATTTAGTATTTTACACATTGGTTTCAGACGGCTCTTAAAGGTCTCT
CTAGCTTCATGTCAACCATCTAAACCAACCACCATTAATGTTCCTCTGCCCATTTTCAAG
AAATTAGAAATCATTTTTCTCTAAGTTCTTGATCGTCGCCATGAACGATCTTATCTCAAG
CTCATTCAAGAGATACACAGACTTAAACCACCAGGTCCAACTCGATGACATCGAGTCTCA
AAATGTTTCTCTTGATTCAGGCAACCTCGATGAGTTCTTTGGATATGTCGAGAGTGTTAA
AGAAGACATGAAAGCTGTCGACGAGATTCATAAGCGTCTCCAAGATGCTAATGAAGAGTC
CAAGACCGTACATGATTCCAAGGCCGTGAAGAAGCTTCGTGCTCGTATGGATTCGAGTGT
TACAGAGGTCTTGAAACGTGTCAAGATGATAAAGACTAAGCTTGTAGCTTTGGAGAAATC
AAATGCTGCGCAGAGGAAAGTCGCTGGTTGTGGTCCTGGCTCGTCTGCTGATCGGACCAG
AACATCGGTCGTTAGTGGATTGGGGAAGAAGCTTAAAGACATGATGGATGATTTTCAGAG
ACTACGAACCAAAATGGCTACTGAATACAAAGAAACGGTCGAGAGAAGGTAAATTTTATT
TCTTTTTCTTTCATTTAGGTGTTACTCTGATCCAACAAAGACCAAATCCTTTTACTATGA
TTGAACAATGATCAAATCTTATTTCAAAAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGNTCATATGGAGAAAAAGAAAGAGTAATTACCAATT
T
Primers used for PCR NR79 - 8474, show primer details
   
Sequence Name 35-K102514-0022-923-G03C-OriL
GenBank Accession LN501784 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102514-0022-923-G03C-OriL
ATTTACATTGAATCATTGTAAATAAGATTCACTGTAACGGGGCAAAAAGCGGACGAAGAG
ACGGTCGAGAAGCTCATATCGAGCGGAGAGAGCGAACGTTTTCTCCAAAAAGCAATACAA
GAGCAAGGTCGCGGACAGGTCATGGACACATTATCAGAGATTCAAGAACGACACGACACG
GTCAAGGAGATAGAACGGAGTCTGTTAGAGCTGCACCAAGTGTTCCTCGACATGGCTGCT
CTTGTCGAAGCTCAAGGGAATATGCTCAATGACATTGAATCAAATGTCTCAAAGGCGAGC
TCTTTCGTCATGAGAGGGACTGATCAATTGCATGGGGCTAAAGTACTTCAGAGGAACAAC
AGGAAATGGGCTTGTATCGCCACTATCCTCGCTATTGTAGTTGTTATTGTGATTCTTTTC
CCAATCCTCTTTAACACTCTACTTCGACCTTGATTTCAAAATGTAACTTATTGTTTTCTC
CAAACTCCTTTTGTTAAGNCCCTTT
   
Sequence Name 54-K102515-0022-923-G03C-NR79
GenBank Accession LN508437 [GenBank]
Sequencing primer gene specific primer, NR79: 5'-GAAAGGGCTCTAACAAAAGGAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K102515-0022-923-G03C-NR79
TTTGAATCAGGTCGAAGTAGAGTGTTAAAGAGGATTGGGAAAAGAATCACAATAACAACT
ACAATAGCGAGGATAGTGGCGATACAAGCCCATTTCCTGTTGTTCCTCTGAAGTACTTTA
GCCCCATGCAATTGATCAGTCCCTCTCATGACGAAAGAGCTCGCCTTTGAGACATTTGAT
TCAATGTCATTGAGCATATTCCCTTGAGCTTCGACAAGAGCAGCCATGTCGAGGAACACT
TGGTGCAGCTCTAACAGACTCCGTTCTATCTCCTTGACCGTGTCGTGTCGTTCTTGAATC
TCTGATAATGTGTCCATGACCTGTCCGCGACCTTGCTCTTGTATTGCTTTTTGGAGAAAA
CGTTCGCTCTCTCCGCTCGATATGAGCTTCTCGACCGTCTCTTCGTCCGCTTTTTGCCCC
GTTACAGTGAATCTTATTTACAATGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11