Sequences from confirmation amplicon


Confirmation sequences for line 924H08 and hit At1g74150 are shown.
Line ID 924H08
Locus At1g74150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27881272
Primers used for PCR 19re - 8474, show primer details
   
Sequence Name 45-K116132-0022-924-H08C-19re
GenBank Accession LN505647 [GenBank]
Sequencing primer gene specific primer, 19re: 5'-GCAAATCTTGATCTTTTTCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116132-0022-924-H08C-19re
GCGTACCGCCCCGCCCGAGGGACAGTCATAGCTGTACAACAGTTGGTGATAACCTTTTTG
TGTTTGGTGGTACTGATGGGACCAAATATCTCAACGATGTGCACATCCTTGATACTTGTA
AGCTCTTCCATCGTTTCTTTTGTTACCCGGTTAGATTAAAAAAATGCTTGTGTTAACTGT
TCCTGGTTTGACGTTTTTGAGTTTGAATCCAATTTCTTTGTTTTTGTAAATGGCTTCATG
TCCGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 20-K116131-0022-924-H08C-8409
GenBank Accession LN495360 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116131-0022-924-H08C-8409
ACAAGCATTTTTTTAATCTAACCGGGTAAACAAAAGAAACGAAGGAAGAGCTTACAAGTA
TCAAGGACGTGCACATCGTTGAGATATTTGGTCCCATCAGTACCACCAAACACAAAAAGG
TTATCACCCAACTGTTGTACAGCTATGACTGTCCCTCGGGCAGGGCGGTACGCCATTGAT
TTCAGGTCGAATCCATATCTGCGTCTCTGCAACAAAATTGCCACAAATGAGACTAGTTTC
AACAATGAAAATTTTGAAAACATGACTACGAGTAAACCACAAAATAGCATCCCAACAAAT
AGTATATATTAAATCTTGAAAAAGGATCATAAAGTCCAAATCATTCAAGTAAGGTATAAC
AAAGAAAGAAACATTGAGTAGGTTACTAAAGGACAAAGGAAAA

Last Updated on 06.12.2018 16:11