Sequences from confirmation amplicon


Confirmation sequences for line 925E10 and hit At5g15520 are shown.
Line ID 925E10
Locus At5g15520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5037853
Primers used for PCR 25pf - 8474, show primer details
   
Sequence Name 71-K115259-0022-925-E10C-25pf
GenBank Accession LN512609 [GenBank]
Sequencing primer gene specific primer, 25pf: 5'-ACAAAGAATGAACAAAGGAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K115259-0022-925-E10C-25pf
GTGCCTTTAGAAAGAATAAGAATTACCCTTTAGTGTCAAGCTCGACAATGCTCATTGTCT
CCAACTGTTGGAGAATATGGCGAGCAATACCACCACTGCTCTTACAGAAATGTGGTGGTC
GACTTCCATTTCTTTTGCTACCTCCATAAATTCTTCTGAAAGCACCAACACCAAGACCTC
CTCTCAAGTAAATCTTTCTGGCCATAGAAGCTGCACCATACAAGATATCAACAACGTTCA
TAACGACTCTATAAAAACAAAAACAAGCGAAGAGAAAGCTAAGCTTTTTTTTACCAGCTC
TAATGTAGTACCAATCAGGGTCATATGGAGCAAGCTCCTTCAAACGACCAGTCTTCACAA
TGTCTGTCCATAGAGGAAGCTCAATCTAAATCAGACAAAAGAGACAACTATTATTACAGT
GAATTTCCAATTAGCTGCCTATGATCACTAACATCAAGAATCTATCATTTCCAATTAAAC
TATCAGTGTTTGACCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAAAT
   
Sequence Name 31-K115258-0022-925-E10C-8409
GenBank Accession LN500287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K115258-0022-925-E10C-8409
CATTTCATTGATATGATGGTCAAACACTGATAGTTTAATTGGAAATGATAGATTCTTGAT
GTTAGTGATCATAGGCAGCTAATTGGAAATTCACTGTAATAATAGTTGTCTCTTTTGTCT
GATTTAGATTGAGCTTCCTCTATGGACAGACATTGTGAAGACTGGTCGTTTGAAGGAGCT
TGCTCCATATGACCCTGATTGGTACTACATTAGAGCTGGTAAAAAAAAGCTTAGCTTTCT
CTTCGCTTGTTTTTGTTTTTATAGAGTCGTTATGAACGTTGTTGATATCTTGTATGGTGC
AGCTTCTATGGCCAGAAAGATTTACTTGAGAGGAGGTCTTGGTGTTGGTGCTTTCAGAAG
AATTTATGGAGGTAGCAAAAGAAATGGAAGTCGACCACCACATTTCTGTAAGAGCAGTGG
TGGTATTGCTCGCCATATTCTCCAACAGTTGGAGACAATGAGCATTGTCGAGCTTGACAC
TAAAGGGTAATTCTTATTTCTTTCTAAAGGCACTTTTCGTAGGAAGACCTTGTTAGTGAC
TTACTTTCATCTCTCCTCCTTTGTTCATTCTTTG

Last Updated on 06.12.2018 16:11