Sequences from confirmation amplicon


Confirmation sequences for line 926H03 and hit At5g53320 are shown.
Line ID 926H03
Locus At5g53320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21637077
Primers used for PCR EU96 - 8474, show primer details
   
Sequence Name 42-K103927-0022-926-H03C-EU96
GenBank Accession LN504438 [GenBank]
Sequencing primer gene specific primer, EU96: 5'-ATCTTCAAAGGACAAACCTAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K103927-0022-926-H03C-EU96
ACGTGGGTGAGGAGATAACAAGATTGTGTTCTTCGAAGGGAAGAATCTTGTCTTTGACCT
AGAAGACTTGTTGAGAGCTTCTGCAGAAGTTTTAGGGAAGGGCCCGTTTGGGACAACCTA
CAAGGTTGATCTCGAGGACTCTGCGACCATCGTTGTGAAAAGGATCAAGGAAGTGAGTGT
ACCTCAACGGGAAT
   
Sequence Name 58-K103928-0022-926-H03C-8409
GenBank Accession LN509687 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K103928-0022-926-H03C-8409
GAGCCAACTTAGCTTGTAAGATACTGTATAAATTCAAATGGTGGCATTATCAACATACCG
TGTAGCAATGTTGAAAGACTTCCATGTTCGTAGTAATCATAAACCACGAGCTTCTCATCC
TTAGAGTAGAAATATCCTCTTAAAGTGGCAACATTCTCATGCTTGATGCTGCCAATATTT
TCGATTTGCTGCTCGAATTCCCGTTGAGGTACACTCACTTCCTTGATCCTTTTCACAACG
ATGGTCGCAGAGTCCTCGAGATCAACCTTGTAGGTTGTCCCAAACGGGCCCTTCCCTAAA
ACTTCGGCAGAAGCTCTCAACAAGTCTTCTAGGTCAAAGACAAGATTCTTCCCTTCGAAC
AACACAATCTTGTCATCTCCTTCACCCTCTTTTGGATCATAATCCTTC
Primers used for PCR CN34 - 8474, show primer details
   
Sequence Name 73-K118414-0022-926-H03C-CN34
GenBank Accession LN512973 [GenBank]
Sequencing primer gene specific primer, CN34: 5'-CATCTCCACCATCTCTTCCTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118414-0022-926-H03C-CN34
CCTCTCCTGTCATTCTTCTCTAACCACCGAGTTCACCCACCTCACCAGGTTTGCCACTTC
TGATTTTCCTGTTAGTACCTCGAATATCAATATCCCGAAACTATACACATCAGACGGTTG
AGTCCCTTTTCTTGTGTCTGTTATCTCTGGTGCACGATAGCCAACTGCATGCCGCGGTAA
TGAATGCATCAGTGTAGCCATTCCTGTTCCAGATATGCAGCCATATCCTTTCCCGTTGAG
GAAAATGTTTGAGGATTTTATGTTCCCGTGGACTAGTTTACCTCCGCTTTGTGAGTGGAT
ATGAGCTACTCCTCTTGCTGTTCCATACACCATATTCAGTCTAGTTTCCCATTCTAGACG
CTTCCGATCCCTGAGACCTTTCTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 25-K118413-0022-926-H03C-8409
GenBank Accession LN497663 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118413-0022-926-H03C-8409
ATTTACAATTGAATATATCAGAAAGGTCTCAGGGATCGGAAGCGTCTAGAATGGGAAACT
AGACTGAATATGGTGTATGGAACAGCAAGAGGAGTAGCTCATATCCACTCACAAAGCGGA
GGTAAACTAGTCCACGGGAACATAAAATCCTCAAACATTTTCCTCAACGGGAAAGGATAT
GGCTGCATATCTGGAACAGGAATGGCTACACTGATGCATTCATTACCGCGGCATGCAGTT
GGCTATCGTGCACCAGAGATAACAGACACAAGAAAAGGGACTCAACCGTCTGATGTGTAT
AGTTTCGGGATATTGATATTCGAGGTACTAACAGGAAAATCAGAAGTGGCAAACCTGGTG
AGGTGGGTGAACTCGGTGGTTAGAGAAGAATGGACAGGAGAGGTGTTTGATGAGGAGCTT
TTGAGGTGTACCCAAGTAGAGGAAGAGAGGTTTGGAGAT

Last Updated on 06.12.2018 16:11