Sequences from confirmation amplicon


Confirmation sequences for line 929A04 and hit At1g06120 are shown.
Line ID 929A04
Locus At1g06120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1857098
Primers used for PCR 26yu - 8474, show primer details
   
Sequence Name 85-K122535-0022-929-A04C-26yu
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 26yu: 5'-CAGTCTTATGCAGCCTACGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K122535-0022-929-A04C-26yu
TATCTTTCTTTCTTTCATAACTTCACTGTCATTGATGTTTCTTCTGTCACTATTGATAAT
ATATATCCAAAGTAATTGAAATTATGACTTCTGGACTCGGATTCTAGATTTTCTTGATTA
ATTTCGTGTCTGGGAAATCTCAGATTCCAACGACGATGAAGATGGAGAAAGCTTGTAACC
CGTTTCTCCGCACTGAGAACACAGATATCCGTCGAGCTTTAGGTATTCCAGAGACAGCAG
ATGAAGCAGAAGCTCTGGGTATTATAAGAAGAGCAAAGGACAATTTCAAAGCTTAGCTAA
AATATAATAAGAATGGTTCCTCTGTACAGTGATGATGCGATCCTGGTTTAATCTGTAATA
AGGTGTTTTGAGTTTTGACCGTGACGTTACGTCTCCTAGTAGTTTTCATGGTCATACTCG
AAATGATTTTTTTTTTCCTTTTGATCAAACGGTCCTAAGTACCCAAGTTTCTACTTTGAT
GAACCCTCGAATTTTGTTATTATTTGTTTTTGGATCTTTTGAAGGGAGTAGCTTTGAAAT
CAAAGTCACAAGAGTTTTCTCTGAAGTTACCGAATTACATCTTTTGAAGTTTTCTGGCAC
AAGGTTCCGTTACTGTTTCAAGATCATGTATTATATCGGTTTTCACTGTTCATACAACAT
GAAATATACTTAGATGGTGTTACATCAACTGGATACAAGTTCTTGAGAACATATCATTCT
TGATAGTTTTATATAAAATAACAAATGATGAGCGAGTGATGATTACACATATAATTAACG
AAATAGATAGATTCATAGATATAGAGCTTGTGACTAACGAGCTAAAGACATTTTTCGCTT
CTGAGCATCGGTAGGCAATTTGACATCTGTGGCTAAACC
   
Sequence Name 58-K122534-0022-929-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K122534-0022-929-A04C-8409
TTGAATATATCCTGTCCCATCATCATCATATATGATATATGAAACATAAACTGGCTGACT
TTGAAGACATATATGATATTTTCTTGGCAACAGGGTGTTGGAGGAGCAATCGGTTACCAT
GCGACTTGGCTCATAAACTCGGCATGCCATATTTGGGGTTCGCGAGCATGGAACACTAAG
GACACCTCTCGTAACATTTGGTACTTAATTTATAGACCTTTAAACCTTAACCGAATCTGA
TAAAGCTTCATCAAAATTAGTATTAAGTGAATGTGGAATGGTGCAGGTGGCTAGGGCCAT
TCACGATGGGAGAGAGCTGGCACAACAACCACCATGCCTTTGAGGCGTCGGCTAGGCACG
GGCTGGAATGGTATCAGGTAGACTTAACGTGGTACCTCATTTGGTTCTTCCAGGTTCTCG
GTTTAGCCACAGATGTCAAATTGCCTACCGATGCTCAGAAGCGAAAAATGTCTTTAGCTC
GTTAGTCACAAGCTCTATATCTATGAATCTATCTATTTCGTTAATTATATGTGTAATCAT
CACTCGCTCATCATTTGTTATTTTATATAAAACTATCAAGAATGATATGTTCTCAAGAAC
TTGTATCCAGTTGATGTAACACCATCTAAGTATATTTCATGTTGTATGAACAGTGAAAAC
CGATATAATACATGATCTTGAAACAGTAACGGAACCTTGTGCCAGAAAACTTCAAAAGAT
GTAATTCGGTAACTTCAGAGAAAACTCTTGTGACTTTGATTTCAAAGCTACTCCCTTCAA
AAGATCCAAAAACAAATAATAACAAAATTCGAGGGTTCATCAAAGTAGAAACTTGGGTAC
TTAGGACCGTTTGATCAAAAGGAAAAAAAAATCATTTCGAGTATGACCATGAAAACTA

Last Updated on 06.12.2018 16:11