Sequences from confirmation amplicon


Confirmation sequences for line 929E04 and hit At1g73150 are shown.
Line ID 929E04
Locus At1g73150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27504816
Primers used for PCR 18kz - 8474, show primer details
   
Sequence Name 25-K116218-0022-929-E04C-18kz
GenBank Accession LN497605 [GenBank]
Sequencing primer gene specific primer, 18kz: 5'-ATCTATGACAAACCCAGTGAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116218-0022-929-E04C-18kz
CGTCTGCAACAGGGACTTGACGTTTGATGAGAAGCGGCAACTAAGTGAAGACCTCCAGGA
TTTACCTTATGACAAGCTAGAGGCAGTTGTGCAGATTATAAAGAAGAGAACTCCAGAACT
CTCTCAACAAGATGATGAGATTGAGTTAGATATTGATAGTCTTGATCTGGAAACACTTTG
GGAGCTTTTCAGATTCGTGACTGAATATAAGGAGAGCTTAAGCAAGAAAAAGGAGGAACA
GGGATTAGACTCAGAAAGAGATGCTGAATCTTTTCAGATAATGTGTGAGTATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGG
TTATTATAAAA
   
Sequence Name 01-K116217-0022-929-E04C-8409
GenBank Accession LN486723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116217-0022-929-E04C-8409
GCATTTCATTGAATCTCCACATTATCTGAAAAGATTCAGCATCTCTTTCTGAGTCTAATC
CCTGTTCCTCCTTTTTCTTGCTTAAGCTCTCCTTATATTCAGTCACGAATCTGAAAAGCT
CCCAAAGTGTTTCCAGATCAAGACTATCAATATCTAACTCAATCTCATCATCTTGTTGAG
AGAGTTCTGGAGTTCTCTTCTTTATAATCTGCACAACTGCCTCTAGCTTGTCATAAGGTA
AATCCTGGAGGTCTTCACTTAGTTGCCGCTTCTCATCAAACGTCAAGTCCCTGTTTGCAG
ACGCCTCTTCTACAAGCTTCTCGGGGACAACAGGTAAAACTGCAGGTTTCACTGGGTTGG
TCATAGATA

Last Updated on 06.12.2018 16:11