Sequences from confirmation amplicon


Confirmation sequences for line 930C08 and hit At5g52250 are shown.
Line ID 930C08
Locus At5g52250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21217786
Primers used for PCR 33uv - 8474, show primer details
   
Sequence Name 44-K117403-0022-930-C08C-33uv
GenBank Accession LN505318 [GenBank]
Sequencing primer gene specific primer, 33uv: 5'-AGTGGACATTTGACTTCTCCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117403-0022-930-C08C-33uv
TTTTATTTATTTATTTTCTAGCGCTAATTAAAACACTATTAGAATTAATTTTCAATTCCC
TACAAAATGTGTATTCGAATTGCAATGATTGAAAAAGTTCTCAATATCACTTACAATTTC
TTCTTAAGAATTGTAGTAACATTCAATCATATTAAAATTAATAACTATAGACAGTATAAA
ATCAACTGAAAAACAGTTTGTATTAATCATGAGCAGAAAAAAATAATATAAATACTGATT
AGCTTTGTTTGCCCGAGAAAATTTCTAAAGCCCCATCGGATCCTCCGGCCACAAGTGTGC
ACCAATCTTCATCCACTTGTCTCAAACACACACTACTAACAAACCGTCGGTCAGATCCAA
ACCGGTTCGTATGACCCAATCCACAAACCCAAACCGGTTCCTCCCACCTCTTATCGTACA
CAAACACTTGGTTATTCTCTGACCCGGACACGACCAAACCACCATGTCTCCAAACCGACA
ACCCAACAAAGTTCCGGCTATTCACATGTAAATGATCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 20-K117402-0022-930-C08C-8409
GenBank Accession LN495389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117402-0022-930-C08C-8409
GCATTTCATTGATCATTTACATGTGAATAGCCGGAACTTTGTTGGGTTGTCGGTTTGGAG
ACATGGTGGTTTGGTCGTGTCCGGGTCAGAGAATAACCAAGTGTTTGTGTACGATAAGAG
GTGGGAGGAACCGGTTTGGGTTTGTGGATTGGGTCATACGAACCGGTTTGGATCTGACCG
ACGGTTTGTTAGTAGTGTGTGTTTGAGACAAGTGGATGAAGATTGGTGCACACTTGTGGC
CGGAGGATCCGATGGGGCTTTAGAAATTTTCTCGGGCAAACAAAGCTAATCAGTATTTAT
ATTATTTTTTTCTGCTCATGATTAATACAAACTGTTTTTCAGTTGATTTTATACTGTCTA
TAGTTATTAATTTTAATATGATTGAATGTTACTACAATTCTTAAGAAGAAATTGTAAGTG
ATATTGAGAACTTTTTCAATCATTGCAATTCGAATACACATTTTGTAGGGAATTGAAAAT
TAATTCTAATAGTGTTTTAATTAGCGCTAGAAAATAAATAAATAAAATGTATCAAGGAGA
GTGTATATATTGACTTGTAAAATGACAAGGAGAAGTCAATTGGTCCACT

Last Updated on 06.12.2018 16:11