Sequences from confirmation amplicon


Confirmation sequences for line 931C06 and hit At4g38850 are shown.
Line ID 931C06
Locus At4g38850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18126349
Primers used for PCR 46qz - 8474, show primer details
   
Sequence Name 47-K116132-0022-931-C06C-46qz
GenBank Accession LN506302 [GenBank]
Sequencing primer gene specific primer, 46qz: 5'-TAGAGAGATTTGGCATGTGATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K116132-0022-931-C06C-46qz
TTAGTAATCTACAACCCAAAGCCACGTGCTTCGTAATTAAAAAGAAAAACCTCAAAAATA
CTTAATCATTTTCTTTCATACGTTCGTTTATATAACAAGTAACTAAACCCCTTTAGATTC
ACAAACTAAAGAAGAATCCAATTTTAAAATCTCAAAGCTTTCTCCAAGACTAAGAAACAT
TTAAGCTTCAGGAAAACATAAGGGAAAATATGGCTTTTTTGAGGAGTTTCTTGGGTGCTA
AGCAAATTATTCGAAGGGAATCATCGTCGACACCAAGAGGATTCATGGCGGTCTATGTAG
GAGAGAATGATCAGAAGAAGAAGAGATATGTGGTGCCGGTTTCATACTTAAATCTGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
T
   
Sequence Name 22-K116131-0022-931-C06C-8409
GenBank Accession LN496262 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116131-0022-931-C06C-8409
TCCTACATAGACCGCCATGAATCCTCTTGGTGTCGACGAAGATTCCCTTCGAATAATTTG
CTTAGCACCCAAGAAACTCCTCAAAAAAGCCATATTTTCCCTTATGTTTTCCTGAAGCTT
AAATGTTTCTTAATTCTTGGAGAAAGCTTTGAGATTTTAAAATTGGATCTTCTTTAGTTT
GTGAATCTAAAGGGGTTTAGTTACTTGTTATATAAACGAACGTATGAAAGAAATGATTAA
GTATTTTTGAGGTTTTTCTTTTTAATTACAGAGCACATGGCTTTGGGTTGTAGATACTAA
ACCAAGAACAAATCAATAAATGGTGTCTGAGAAGTTAGTGTCTAATGATGTCCTACATGA
TAACTTCATTGGGGCTTATTTGTCTCAAAGACATCACATGC

Last Updated on 06.12.2018 16:11