Sequences from confirmation amplicon


Confirmation sequences for line 931H12 and hit At2g44410 are shown.
Line ID 931H12
Locus At2g44410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18328534
Primers used for PCR BK20 - 8474, show primer details
   
Sequence Name 89-K104544-0022-931-H12C-BK20
GenBank Accession LN514954 [GenBank]
Sequencing primer gene specific primer, BK20: 5'-TTCGAGTGTGTTTGCGTTTAATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K104544-0022-931-H12C-BK20
TATATTTGTTATTTAATAAATATATAATGGTTGGTCAGCATGCCAAGAAAAATATATATT
TTCTAATTAATATTCTTTATAATTTATTTTCTTACAACATAAATCAAACAATTTAACTGA
CGCAACCACAAACCTTAAACATTTGGAAGACAATCATGTCCTAAGCCAGAGATTAAAAAA
AGTTTTTGGTTTAAATATCCAAAGACCTACTTCTTCTATCGGTGTCTCTCTCTCTCTCTC
TCGCCACCGATCATCATGGTCTGCACCGCCTTTGAAATCTACGTCATCCTTTGATCTCTA
GCTCTCTCCAAAGGTCTCTTCTTTCTCGTCAAACTTCATATTAAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 95-K104545-0022-931-H12C-8409
GenBank Accession LN515352 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K104545-0022-931-H12C-8409
GCCATTTACAATTTAATATGAAGTTTGACGAGAAAGAAGAGACCTTTGGAGAGAGCTAGA
GATCAAAGGATGACGTAGATTTCAATGGCGGTGCAGACCATGATGATCGGTGGCGAGAGA
GAGAGAGAGAGACACCGATAGAAGAAGTAGGTCTTTGGATATTTAAACCAAAAACTTTTT
TTAATCTCTGGCTTAGGACATGATTGTCTTCCAAATGTTTAAGGTTTGTGGTTGCGTCAG
TTAAATTGTTTGATTTATGTTGTAAGAAAATAAATTATAAAGAATATTAATTAGAAAATA
TATATTTTTCTTGGCATGCTTGACCAACCATTATATATTTATTTAAATTAAACAAAATTA
ATTAACATATCTTGACTATTAAACGCAAACACACTCG

Last Updated on 06.12.2018 16:11