Sequences from confirmation amplicon


Confirmation sequences for line 932F10 and hit At4g29120 are shown.
Line ID 932F10
Locus At4g29120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14351176
Primers used for PCR 21yp - 8474, show primer details
   
Sequence Name 94-K121557-0022-932-F10C-21yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21yp: 5'-CTATCTTCTTCACCACTAGCACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K121557-0022-932-F10C-21yp
TTTTCTAAACCAGGATTACAAACCCGACGTCGTTAAAGTACTTTGACGAAAAACACCTCC
GAAGAAACTCAAAATTACGATGGTATACCACTCAACTTTCGATAACTACTAGAATCCATC
AATCACCACAGGAAACAATGCCACTTCTCCTCCGTCGATTTCCCTCTCCCTCCGTCGTCT
CCTCCTTCTTCCTCCGTCGCTCAATGGCTTCCTCAACAATCTCCTCGGATATAATCACTC
CATCAAACACCAAAATCGGATGGATCGGAACCGGAGTCATGGGCCGATCAATGTGTGGAC
ACTTAATCAAAGCCGGTTACACAGTCACCGTCTTCAATCGAACCATCTCCAAAGCTCAAA
CTCTCATCGACATGGGTGCTAACGTCGCCGACTCACCAAACTCAGTCGCTGAACAATCCG
ACGTCGTTTTCACCATCGTTGGTTATCCTTCCGATGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAAAGTAGATGTCCGCA
   
Sequence Name 79-K121556-0022-932-F10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K121556-0022-932-F10C-8409
CGGAAGGATAACCAACGATGGTGAAAACGACGTCGGATTGTTCAGCGACTGAGTTTGGTG
AGTCGGCGACGTTAGCACCCATGTCGATGAGAGTTTGAGCTTTGGAGATGGTTCGATTGA
AGACGGTGACTGTGTAACCGGCTTTGATTAAGTGTCCACACATTGATCGGCCCATGACTC
CGGTTCCGATCCATCCGATTTTGGTGTTTGATGGAGTGATTATATCCGAGGAGATTGTTG
AGGAAGCCATTGAGCGACGGAGGAAGAAGGAGGAGACGACGGAGGGAGAGGGAAATCGAC
GGAGGAGAAGTGGCATTGTTTCCTGTGGTGATTGATGGATTCTAGTAGTTATCGAAAGTT
GAGTGGTATACCATCGTAATTTTGAGTTTCTTCGGAGGTGTTTTTCGTCAAAGTACTTTA
ACGACGTCGGGTTTGTAATCCTGGTTTAGAAAATTGATTTGGTTCGGTTAAACTTGGTTT
ATTAGGCTCAAGCGTGCTAGTGGTGAAGAAGATAG

Last Updated on 06.12.2018 16:11