Sequences from confirmation amplicon


Confirmation sequences for line 932G05 and hit At1g13880 are shown.
Line ID 932G05
Locus At1g13880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4750557
Primers used for PCR 42sf - 8474, show primer details
   
Sequence Name 36-K116491-0022-932-G05C-42sf
GenBank Accession LN502365 [GenBank]
Sequencing primer gene specific primer, 42sf: 5'-TTCATTTACTTCCCTCTGGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116491-0022-932-G05C-42sf
GATTCGTCGATATGTTTGAGTTGGAACAGATCAATGTTCTTAGTCCACAATGATCTGGCA
GTATTGAATCACAAGGATCAAATGAATACGACTGATCCACAAGATCATTGCAGTGGCGCC
AGTAATTCCCAATGTTGGATTCTTCTCTATTTCTAACTGGACATCGAGAGTAGATTGCGT
TGGACTGATTCTCAAAGGACATGTACGAATCTTCATTAGCATTAACCTCTTCTCCTTCGT
CTCTTGAGAGGCTTTTTTCGGCTTTGTCATCTGATGGAGTTTCAGCACCATAAAAGGTGT
TGTCATACTCTACTTGCCACTCATCATCATCACTATCAACATCCAGGCTTTTAGAACGGT
TCTGGATAGCTCGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACGCACGG
TTATTAT
   
Sequence Name 04-K116490-0022-932-G05C-8409
GenBank Accession LN488070 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116490-0022-932-G05C-8409
AACCGTTCTAAAGCCTGGATGTTGATAGTGATGATGATGAGTGGCAAGTAGAGTATGACA
ACACCTTTTATGGTGCTGAAACTCCATCAGATGACAAAGCCGAAAAAAGCCTCTCAAGAG
ACGAAGGAGAAGAGGTTAATGCTAATGAAGATTCGTACATGTCCTTTGAGAATCAGTCCA
ACGCAATCTACTCTCGATGTCCAGTTAGAAATAGAGAAGAATCCAACATTGGGAATTACT
GGCGCCACTGCAATGATCTTGTGGATCAGTCGTATTCATTTGATCCTTGTGATTCAATAC
TGCCAGATCATTGTTGGACTAAGAACATTGATCTGCTTCCAACTTCAAACATTATCGACG
AAATCTTTGGTCAAGACCACCCATGGGATGATTTCTCCAGAGGGAGNTAAATGAA

Last Updated on 06.12.2018 16:11