Sequences from confirmation amplicon


Confirmation sequences for line 935F05 and hit At2g02620 are shown.
Line ID 935F05
Locus At2g02620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:718226
Primers used for PCR 40pf - 8474, show primer details
   
Sequence Name 75-K115259-0022-935-F05C-40pf
GenBank Accession LN513289 [GenBank]
Sequencing primer gene specific primer, 40pf: 5'-GCACATAATATTATTTATTATTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K115259-0022-935-F05C-40pf
CGACATACCCTTTTTCATAGACCAACCAAAAAGGCATGATCATACACTCACCCTTTTCCC
TAGACAAGCTTCCTTAACCTGCAATGTTTGTGGTTTAGTTAATAAACTTCATCTCACCTA
CGTCTGCTCCATATGCAATTTTGCAGCCCATAGTGATTGTATCCATATCCCACAAACCAT
AAGAATGTCTCGCCACGACCATCGTATCTCTTTCACTTCTTCTCTTCCATTAGGAATATG
GTCTTGTGGAGTTTGCCGCAAAGAGATTGATGGTGATTATGGTGTAAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCATTATTATA
   
Sequence Name 35-K115258-0022-935-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115258-0022-935-F05C-8409
AAAATGGCTTCNTGTCCTGCAAATCTACATGGATCAACAATGAGTATTATTGTCAATATG
CACAAAAATAAAGAGTAATTACCGATTTTTTTTCACTTCAGAAATGTTGATG

Last Updated on 06.12.2018 16:11