Sequences from confirmation amplicon


Confirmation sequences for line 936C01 and hit At3g59580 are shown.
Line ID 936C01
Locus At3g59580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22012294
Primers used for PCR 11ux - 8474, show primer details
   
Sequence Name 81-K117671-0022-936-C01C-11ux
GenBank Accession LN514111 [GenBank]
Sequencing primer gene specific primer, 11ux: 5'-TTCATGGAGATCAGAATCTGTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K117671-0022-936-C01C-11ux
TTCACTGAAAGGCACCTTCTTGCAGCTTGAAACGCTTAGCCACTTCTCTGTAGAGCTGAG
AACACCCAACAACGTATGGATCAAGCTTGAAACGTACAGTGTCCTCTCTGTAAGTGGCTT
TTACGGTTAGTGTTGAACTTGATCCGCTTTCACCGCTATTGTTATGAGTTCTTATTTGGT
TCCAGTTTTGTTCCAAGGAAGCAGATGAACTTCCCAGCAAAACTGCTCCTGAGCTATTTG
ATGAATCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 33-K117670-0022-936-C01C-8409
GenBank Accession LN501166 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117670-0022-936-C01C-8409
TTGAATAATCCCGGATTCATCAAATAGCTCAGGAGCAGTTTTGCTGGGAAGTTCATCTGC
TTCCTTGGAACAAAACTGGAACCAAATAAGAACTCATAACAATAGCGGTGAAAGCGGATC
AAGTTCAACACTAACCGTAAAAGCCACTTACAGAGAGGACACTGTACGTTTCAAGCTTGA
TCCATACGTTGTTGGGTGTTCTCAGCTCTACAGAGAAGTGGCTAAGCGTTTCAAGCTGCA
AGAAGGTGCCTTTCAGTTGAAATACTTGGATGATGAAGAAGAATGGGTGATGTTGGTCAC
AGATTCTGATCTCCATGAA

Last Updated on 06.12.2018 16:11