Sequences from confirmation amplicon


Confirmation sequences for line 937D05 and hit At3g10185 are shown.
Line ID 937D05
Locus At3g10185 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3145665
Primers used for PCR 57qi - 8474, show primer details
   
Sequence Name 18-K115694-0022-937-D05C-57qi
GenBank Accession LN494443 [GenBank]
Sequencing primer gene specific primer, 57qi: 5'-ATTCCATGCATAAACAAAATCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115694-0022-937-D05C-57qi
TCTTAATAATTCTGGTGTTAATCAGTAATTAAGTATTTTAAAATTAGTTTTTAGTTTTTC
ATATGATTAGTGATTGGTTGTTCAATCCATGAATTTCATTATCGTTAGACCGTTGAAAAG
TTTTATAAAAAAACAAGAGTATATTTAGACAATAATTTTATTAATGGCTTCTACTCTTCT
AGTTTGCAAAAAAATCAGTGACAGGAGAAATATATTTTCTAGATTAAAAAGTATTTGGAA
ATTGGACTATATATAGTGGACCCCTATCATTTGTTCATAATGAGCTTAGTCTGCAAAATA
TATAATCTACCTGAGCTTCATTACAATCATTTCTGTTTTCAGAAAATGGCAACAAAACTT
AGCATCATTGTTTTCTCCATTGTTGTGTTACATCTTCTTCTGTCTGCCCATATGCATGTA
AGTGTTTCAACAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATA
   
Sequence Name 09-K115693-0022-937-D05C-8409
GenBank Accession LN490328 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115693-0022-937-D05C-8409
ATTTCAATTGAATAATACTCATTGTTGAAACACTTACATGCATATGGGCAGACAGAAGAA
GATGTAACACAACAATGGAGAAAACAATGATGCTAAGTTTTGTTGCCATTTTCTGAAAAC
AGAAATGATTGTAATGAAGCTCAGGTAGATTATATATTTTGCAGACTAAGCTCATTATGA
ACAAATGATAGGGGTCCACTATATATAGTCCAATTTCCAAATACTTTTTAATCTAGAAAA
TATATTTCTCCTGTCACTGATTTTTTTGCAAACTAGAAGAGTAGAAGCCATTAATAAAAT
TATTGTCTAAATATACTCTTGTTTTTTTATAAAACTTTTCAACGGTCTAACGATAATGAA
ATTCATGGATTGAACAACCAATCACTAATCATATGTAAAAACTAAAAACTAATTTTAAAA
TACTTAATTACTGATTAAACACCAGAATTATGTAAGATATACTACATTTCCCCTAAAAAA
TATCTGAAGTTGTGATTTTGTTTATGCATGGAATA

Last Updated on 06.12.2018 16:11