Sequences from confirmation amplicon


Confirmation sequences for line 938A03 and hit At2g35690 are shown.
Line ID 938A03
Locus At2g35690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15000659
Primers used for PCR 53kk - 8474, show primer details
   
Sequence Name 81-K113595-0022-938-A03C-53kk
GenBank Accession LN514094 [GenBank]
Sequencing primer gene specific primer, 53kk: 5'-AAACTTCATTCCGATATCACCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K113595-0022-938-A03C-53kk
TCCAACTACGCAGCTGCACGATGAATCCTGAAAAACAGAAAAATCTTGTTACTCAATACT
ACTGATGATTATTAAAAAAATGAATAAGAAATCGTTCTAGTGCCTTACCATGTACACCAT
GGTCTTTGCCATTGGTTATTAGACGAGCATAAATAACAGCATGAGTAGAAACTTTTCCTA
ACCCACCAGGCCACCACTAATGTTTCAAACAAAACAAAATCAAGACACAATTTAAACACT
CAGAGATCAAAATAGATAACCAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 39-K113594-0022-938-A03C-8409
GenBank Accession LN503421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K113594-0022-938-A03C-8409
ATTTCATTGGTAATTGGTTATCTATTTTGATCTCTGAGTGTTTAAATTGTGTCTTGATTT
TGTTTTGTTTGAAACATTAGTGGTGGCCTGGTGGGTTAGGAAAAGTTTCTACTCATGCTG
TTATTTATGCTCGTCTAATAACCAATGGCAAAGACCATGGTGTACATGGTAAGGCACTAG
AACGATTTCTTATTCATTTTTTTAATAATCATCAGTAGTATTGAGTAACAAGATTTTTCT
GTTTTTCAGGATTCATCGTGCAGCTGCGTAGTTTGGATGATCATTCTCCTCTTCCGGGTA
TAACCGTTGGTGATATCGGAATGAAGTTTA

Last Updated on 06.12.2018 16:11