Sequences from confirmation amplicon


Confirmation sequences for line 938A08 and hit At1g54000 are shown.
Line ID 938A08
Locus At1g54000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20155007
Primers used for PCR 60wj - 8474, show primer details
   
Sequence Name 29-K118380-0022-938-A08C-60wj
GenBank Accession LN499480 [GenBank]
Sequencing primer gene specific primer, 60wj: 5'-CAACACCTAATTCAAAGAAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118380-0022-938-A08C-60wj
AGCGGGATTCTTTTCGTTTTACTTTCACAGAAAAGCATGTGACTTAATATCAATAAGCTT
TACTTTAATTGCACTTATAATATTGGTTTAATTTTTGTCTTTTTCTCTTTTTTGGTTCTT
TAGATGACTCTGAATCAACAAGTGAAAAAATTCAATCAAATGAAAGCAGCAAATTGGAAC
GATGATTTCGTGGCAAAGTCAGTATTTATGATATACATTGGTGCAAACGATTACTTAAAT
TTCACCAAGAACAACCCTACCGCTGATGCATCTGCTCAACAAGCTTTTGTTACTTCCGTG
ACCAACAAGTTAAAGAACGATATTAGCGCGTTGTATTCATCAGGAGCGAGTAAATTCGTG
ATCCAAACCTTAGCTCCATTGGGTTGCTTACCCATCGTCAGACAAGAATACAACACCGGG
ATGGATCAATGCTATGAGAAACTCAACGATTTGGCTAAACAACACAACGAAAAAATTGGA
CCGATGTTGAACGAAATGGCTAGAAACTCTCCTGCATCAGCTCCTTTCCAATTCACCGTC
TTTGATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCACAAATGTAGA
   
Sequence Name 19-K118379-0022-938-A08C-8409
GenBank Accession LN494961 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118379-0022-938-A08C-8409
CCTGGAAATCAAAGACGGTGAATTGGAAAGGAGCTGATGCAGGAGAGTTTCTAGCCATTT
CGTTCAACATCGGTCCAATTTTTTCGTTGTGTTGTTTAGCCAAATCGTTGAGTTTCTCAT
AGCATTGATCCATCCCGGTGTTGTATTCTTGTCTGACGATGGGTAAGCAACCCAATGGAG
CTAAGGTTTGGATCACGAATTTACTCGCTCCTGATGAATACAACGCGCTAATATCGTTCT
TTAACTTGTTGGTCACGGAAGTAACAAAAGCTTGTTGAGCAGATGCATCAGCGGTAGGGT
TGTTCTTGGTGAAATTTAAGTAATCGTTTGCACCAATGTATATCATAAATACTGACTTTG
CCACGAAATCATCGTTCCAATTTGCTGCTTTCATTTGATTGAATTTTTTCACTTGTTGAT
TCAGAGTCATCTAAAGAACCAAAAAAGAGAAAAAGACAAAAATTAAACCAATATTATAAG
TGCAATTAAAGTAAAGCTTATTGATATTAAGTCACATGCTTTTCTGTGAAGTAAAACAAA
AAGAATCCCGCTTAGTATGACCGGGTCAATAATAGACAGATATAATTGATTTT

Last Updated on 06.12.2018 16:11