Sequences from confirmation amplicon


Confirmation sequences for line 938B09 and hit At4g19130 are shown.
Line ID 938B09
Locus At4g19130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10467215
Primers used for PCR 02sd - 8474, show primer details
   
Sequence Name 40-K116218-0022-938-B09C-02sd
GenBank Accession LN503830 [GenBank]
Sequencing primer gene specific primer, 02sd: 5'-CTATAGTGATTCCCAGATTGACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116218-0022-938-B09C-02sd
TTCTTGGACGATGAGGGATCTCTGGTTCCTGAGCTTCCTATACCAGTATTAACCACATCA
GCATTGTAATCGGATCCTTCTGGGTTAATGGGAAGAGAATTTGCATCTCTGGTCCTGAGT
TTATCCATAGCACCTAACATAGTCCTGGTATCTGCTGAATAGTTTAGCTTGTCCACTTTC
ACAACTGTTGCTTTCACACGCTGTTCGTCGCTGAATGTTTCTTCTTTTACCTTCAGCTTG
AAGTTGTACTTGGTAAAAGCAACCTTACGGATGATATCTTCGAATTTTTCCTCATCCTTG
TGTTCATTCTTCACATAATACAAATCTTTAGCAGATATGCCCATTATCTCTTCACCAGCC
TCCTGGAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAAGGTTATTAT
   
Sequence Name 16-K116217-0022-938-B09C-8409
GenBank Accession LN493578 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116217-0022-938-B09C-8409
ATTTCATTGATTCCGGAGGCTGGTGAAGAGATAATGGGCATATCTGCTAAAGATTTGTAT
TATGTGAAGAATGAACACAAGGATGAGGAAAAATTCGAAGATATCATCCGTAAGGTTGCT
TTTACCAAGTACAACTTCAAGCTGAAGGTAAAAGAAGAAACATTCAGCGACGAACAGCGT
GTGAAAGCAACAGTTGTGAAAGTGGACAAGCTAAACTATTCAGCAGATACCAGGACTATG
TTAGGTGCTATGGATAAACTCAGGACCAGAGATGCAAATTCTCTTCCCATTAACCCAGAA
GGATCCGATTACAATGCTGATGTGGTTAATACTGGTATAGGAAGCTCAGGAACCAGAGAT
CCCTCATCGGTCCAAAGAAGGGATTTCGGTTTACATGCGCATCAATCGGGTCAATCTGGG
ATTCACTATAGA

Last Updated on 06.12.2018 16:11