Sequences from confirmation amplicon


Confirmation sequences for line 941B10 and hit At1g08465 are shown.
Line ID 941B10
Locus At1g08465 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2679153
Primers used for PCR 12ux - 8474, show primer details
   
Sequence Name 58-K117566-0022-941-B10C-12ux
GenBank Accession LN509816 [GenBank]
Sequencing primer gene specific primer, 12ux: 5'-TCATCAGCTCTCTGCTTACACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117566-0022-941-B10C-12ux
ATCATACACACGCATATATATCTATACACATATATATACGTATAAGTGTATATATGTGTG
TATATATATAAAGGCGTGAGTAATAGCCATTAGACTTTTGGCCTGCAACTGACTGGTCTA
ATTGCTTGCCTTTCTTGTTGCCATCCAGCTTTAATCCAAAGTGAATGTGAGGAAAATGTG
CCCACTACACCCAAAAATTGAAAGGCAAATTTATTTTTAAAAAACATCTAATACTGTGAT
ATATAAACATTCCACCAATTATAAATAGTAGAACCACTATCTTATTGGTATACATACACA
CTTATACCACAGTTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGATTATTATAAAA
   
Sequence Name 26-K117565-0022-941-B10C-8409
GenBank Accession LN498109 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117565-0022-941-B10C-8409
CCATTTAAACTGTGGTATAAGTGTGTATGTATACCAATAAGATAGTGGTTCTACTATTTA
TAATTGGTGGAATGTTTATATATCACAGTATTAGATGTTTTTTAAAAATAAATTTGCCTT
TCAATTTTTGGGTGTAGTGGGCACATTTTCCTCACATTCACTTTGGATTAAAGCTGGATG
GCAACAAGAAAGGCAAGCAATTAGACCAGTCAGTTGCAGGCCAAAAGTCTAATGGCTATT
ACTAAAGCCTTTATATATATACACACATATATACACTTATACGTATATATATGTGTATAG
ATATATATGCGTGTGTATGATTTATGTTTATCTTTGTACGTACGCATTAATGAATGTGTA
AGCAGAGAGCTGATGAAAT

Last Updated on 06.12.2018 16:11