Sequences from confirmation amplicon


Confirmation sequences for line 941C06 and hit At2g32610 are shown.
Line ID 941C06
Locus At2g32610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13838438
Primers used for PCR 11me - 8474, show primer details
   
Sequence Name 26-K114053-0022-941-C06C-11me
GenBank Accession LN498023 [GenBank]
Sequencing primer gene specific primer, 11me: 5'-GTAAATTGAACTTCATTTAAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114053-0022-941-C06C-11me
CACCTTGGGGATAAGGTAGAGTTGTGGAGTAGGCAATAAGCAGGAAGGAGACAATAAATA
AGCTCAGGGATTGACCTTAGACAGGTGATAATGCAAAGATAAGCTAATCGTTGTCGGAAT
CTTATTTTCTTGCTAAACAATCCTCGCAACGGACTTTGCTTGTTGAAAAGGATTTCGATC
CATCCTGTCGCCCATCGACGCTGCTGGAGTAACGCCTCGGGTACTCCTGCCGGCATAGAT
CCAAGAAATGCAGGTGTATCCGGAGAAATGTATGAGCTAGTCCAACCTCTCGAATGTATT
CCGATACTCGTGTTTAAATCTTCCGCCACTGAATCATATAACCAGCCGATCTGCATAACT
TTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 10-K114052-0022-941-C06C-8409
GenBank Accession LN490748 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114052-0022-941-C06C-8409
ATTGATATTGAAAGTTATGCAGATCGGCTGGTTATATGATTCAGTGGCGGAAGATTTAAA
CACGAGTATCGGAATACATTCGAGAGGTTGGACTAGCTCATACATTTCTCCGGATACACC
TGCATTTCTTGGATCTATGCCGGCAGGAGTACCCGAGGCGTTACTCCAGCAGCGTCGATG
GGCGACAGGATGGATCGAAATCCTTTTCAACAAGCAAAGTCCGTTGCGAGGATTGTTTAG
CAAGAAAATAAGATTCCGACAACGATTAGCTTATCTTTGCATTATCACCTGTCTAAGGTC
AATCCCTGAGCTTATTTATTGTCTCCTTCCTGCTTATTGCCTACTCCACAACTCTACCTT
ATTCCCCAAGGTGACTCTCTCTCTGACACTCATAATAAGGTTTAAAGTTCTGTTTTGGTT
TAAATGAATTCCAATTTA

Last Updated on 06.12.2018 16:11