Sequences from confirmation amplicon


Confirmation sequences for line 941G01 and hit At3g28810 are shown.
Line ID 941G01
Locus At3g28810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10822743
Primers used for PCR 16hk - 8474, show primer details
   
Sequence Name 28-K112877-0022-941-G01C-8409
GenBank Accession LN498922 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112877-0022-941-G01C-8409
TTTCATTGAATTATCCTGTCCACAGGTAAAAAGTCTATGGAAACTTACCAAGAGCAAGTG
ATGAAAACTCTTCAAGAGTTAGATACCATCCACAGTCAAATTGTGAGTCAGAACCAAGGC
AAGAAAGAAGGATCTTTGACATGCACACCAGCACAACAAACACAGATTAAGACAACAATT
ACTAAGTGGGAACAAGTCACAACCCAATTCGTAGAGACCGCTATTCAGAGCGAGACAAAG
TCATCATCAACTACTTCATCTTCTGTTGGCAAAATGAACACAAATTGAAATCAAAGGCAT
AACTAAAATAATGCCCTATGTTAATACTACTTTTGTTTTTGTTGTAAATAAAACCAATAT
CTGTATAGGCCAGCCATAGATCAGTCGAGCGATCTATACAAGGAATAACTATGTAAATGT
AAATATATATTGTAACAAACAGTCAGAAGTGAGTGAACTCACTCGATGATTTTTATTGAT
ATTTTTGTCTTCTTCGAAATTAAACTAAGAAGAATATGTTTAAGACCATAAAAGGCAACG
AACATAA
   
Sequence Name 65-K112878-0022-941-G01C-16hk
GenBank Accession LN511434 [GenBank]
Sequencing primer gene specific primer, 16hk: 5'-ATTATGTCGTTTGCCTTTTATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K112878-0022-941-G01C-16hk
AAATATCAATAAAAATCATCGAGTGAGTTCACTCACTTCTGACTGTTTGTTACAATATAT
ATTTACATTTACATAGTTATTCCTTGTATAGATCGCTCGACTGATCTATGGCTGGCCTAT
ACAGATATTGGTTTTATTTACAACAAAAACAAAAGTAGTATTAACATAGGGCATTATTTT
AGTTATGCCTTTGATTTCAATTTGTGTTCATTTTGCCAACAGAAGATGAAGTAGTTGATG
ATGACTTTGTCTCGCTCTGAATAGCGGTCTCTACGAATTGGGTTGTGACTTGTTCCCACT
TAGTAATTGTTGTCTTAATCTGTGTTTGTTGTGCTGGTGTGCATGTCAAAGATCCTTCTT
TCTTGCCTTGGTTCTGACTCACAATTTGACTGTGGATGGTATCTAACTCTTGAAGAGTTT
TCATCACTTGCTCTTGGTAAGTTTCCATAGACTTTTTAACCTGTGGACAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCA
CAGCGNTTATAA

Last Updated on 06.12.2018 16:11