Sequences from confirmation amplicon


Confirmation sequences for line 942B08 and hit At4g26470 are shown.
Line ID 942B08
Locus At4g26470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13371682
Primers used for PCR 36pq - 8474, show primer details
   
Sequence Name 54-K115259-0022-942-B08C-36pq
GenBank Accession LN508576 [GenBank]
Sequencing primer gene specific primer, 36pq: 5'-GAAGAAGAGGGAGGGAGTTTAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115259-0022-942-B08C-36pq
TCTCCTGATTCATCAATGGCTCGGACCATCTCCTCGCGGCTAACATATCCGTCCTTGTTC
TCGTCTAGGAACACAAATGTGTCAACAAGCGTCTCGAATGTTGGTTCCAGCTTTGGCATT
CCCATTGTCCATTTCTATTCATCACACAAAAATAGCTTTAAAACCATTGAATAACCAATC
AAAATCTAAAACGAAATATCAAACCGAGCATAGGAGATCGATAAAAGTATTTTGCGGGTA
CCTTTTGGAGAGTGGATGAATCGTCTTTAAGAAGATAGACGAGGCAAAGAAGAACAATGA
ATTCTGTAAATGTAATTCCCATATCCTCGTTAATATCGCAAGCCTTGAACAAATCATTAA
TCTCTTCTTCATCGAACGAGATCTCTAGCTTCCTGATACAATTCTTTAGCTCCGTGTGAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCC
   
Sequence Name 14-K115258-0022-942-B08C-8409
GenBank Accession LN492636 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115258-0022-942-B08C-8409
ATTGAATATCCACGGAGCTAAAGAATTGTATCAGGAAGCTAGAGATCTCGTTCGATGAAG
AAGAGATTAATGATTTGTTCAAGGCTTGCGATATTAACGAGGATATGGGAATTACATTTA
CAGAATTCATTGTTCTTCTTTGCCTCGTCTATCTTCTTAAAGACGATTCATCCACTCTCC
AAAAGGTACCCGCAAAATACTTTTATCGATCTCCTATGCTCGGTTTGATATTTCGTTTTA
GATTTTGATTGGTTATTCAATGGTTTTAAAGCTATTTTTGTGTGATGAATAGAAATGGAC
AATGGGAATGCCAAAGCTGGAACCAACATTCGAGACGCTTGTTGACACATTTGTGTTCCT
AGACGAGAACAAGGACGGATATGTTAGCCGCGAGGAGATGGTCCGAGCCATTGATGAATC
AGGAGAACGATCCTCCGGGCGGATTGCAATGAAGAGATTTGGTTTATTCTTAAACTCCCT
CAATCTTCT

Last Updated on 06.12.2018 16:11