Sequences from confirmation amplicon


Confirmation sequences for line 942G03 and hit At1g01960 are shown.
Line ID 942G03
Locus At1g01960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:336818
Primers used for PCR 07oi - 8474, show primer details
   
Sequence Name 26-K114782-0022-942-G03C-07oi
GenBank Accession LN498047 [GenBank]
Sequencing primer gene specific primer, 07oi: 5'-GGGTTATAATAATGCAAGAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114782-0022-942-G03C-07oi
CACCGGCATTCTCAGCAGAATCTTCAATAACTCGAGAGCCACAATCTTCCCTCTCATCAA
CTCTGGATCTTCCTTGGGAGGCGTCTTCATGGACAGCTTGCAAAGTGCCCTGAAAACCAA
GAAGGCGTCTCTTCTCAGCTTATTCCCAATCTGAACTTCAGAATCATCGTCTTTCTCCAC
TTCTCCATCGGCCAGCTCTCCTTTTCTTCCCTCGAGGGCCGACTTATACATACTGATCTC
CCAGTACTTGGCATCCAACATATCCTTATCAGTAGAATCCAACAAATCAGTAGGATTGGC
AGTGCCAGGCAATGAAGTCTCGAAGGCCCCGTCATGGCCGCCGAATGTGCCCTTGGCGTT
AGCTGAATTGAAGACTCCGTCAATGTCCTGCATAATCTTGGTAATGAAACCTTGAACAGC
AATCTACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCA
   
Sequence Name 26-K114769-0022-942-G03C-8409
GenBank Accession LN498046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114769-0022-942-G03C-8409
CGGAGTTCTTTAATTCCAGCTAACGCCAAGGGCACATTCGGCGGCCATGACGGGGCCTTC
GAGACTTCATTGCCTGGCACCGCCAATCCTACTGATTTGTTGGATTCTACTGATAAGGAT
ATGTTGGATGCCCAGTTACTGGGAGATCAGTATGTATAAGTCGGCCCTCGAGGGAAGAAA
AGGAGAGCTGGCCGATGGAGAAGTGGAGAAAGACGATGATTCTGAAGTTCAGATTGGGAA
TAAGCTGAGAAGAGACGCCTTCTTGGTTTTCAGGGCACTTTGCAAGCTGTCCATGAAGAC
GCCTCCCAAGGAAGATCCAGAGTTGATGAGAGGGAAGATTGTGGCTCTCGAGTTATTGAA
GATTCTGCTTGAGAATGCCGGTGCTGTCTTTAGAACCAGCGATAGGTTTGTTTTGTTTAT
CTTC

Last Updated on 06.12.2018 16:11