Sequences from confirmation amplicon


Confirmation sequences for line 943C08 and hit At2g16850 are shown.
Line ID 943C08
Locus At2g16850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7303136
Primers used for PCR 22vy - 8474, show primer details
   
Sequence Name 12-K118356-0022-943-C08C-22vy
GenBank Accession LN491793 [GenBank]
Sequencing primer gene specific primer, 22vy: 5'-CATATGTGAGTGGTTTATGATCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118356-0022-943-C08C-22vy
GTACATAGACAAAAACACCAAGGAATGTTTTATACGAACATGCATGTGGGTTCATATAAA
CTTGTAACAAATGTTTTAATTCGTACTAAAGTATAAAAGTGAATCATAAGATGCCCTACA
TGAAAGAAAGCAACGGACAAAGATGAATTAAACCAAATTAAACTATGAAAATGAATAAAT
CCGATTACATCAAATTAAAACACACAAATATCTCTCATTAAGCACACAAAAAAAAAGATC
TCAAATTGTTCTAAGAAGTCTCATGAATCATCAATTGGTTGGGTTGCTGCGGAATGAAGC
CAAAGCTTTGATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K118355-0022-943-C08C-8409
GenBank Accession LN491792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118355-0022-943-C08C-8409
TACATTGAAATATCCTGCAATCAAAGCTTTGGCTTCATTCCGCAGCAACCCAACCAATTG
ATGATTCATGAGACTTCTTAGAACAATTTGAGATCTTTTTTTTTGTGTGCTTAATGAGAG
ATATTTGTGTGTTTTAATTTGATGTAATCGGATTTATTCATTTTCATAGTTTAATTTGGT
TTAATTCATCTTTGTCCGTTGCTTTCTTTCATGTAGGGCATCTTATGATTCACTTTTATA
CTTTAGTACGAATTAAAACATTTGTTACAAGTTTATATGAACCCACATGCATGTTCGTAT
AAAACATTCCTTGGTGTTTTTGTCTTATGTTACTTGATTCTTCTTATCCGGTGGTCGGAT
CATAAACCCTCCACATATG

Last Updated on 06.12.2018 16:11