Sequences from confirmation amplicon


Confirmation sequences for line 944B03 and hit At5g55520 are shown.
Line ID 944B03
Locus At5g55520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22488230
Primers used for PCR 58kh - 8474, show primer details
   
Sequence Name 78-K113845-0022-944-B03C-58kh
GenBank Accession LN513720 [GenBank]
Sequencing primer gene specific primer, 58kh: 5'-TCATATCATTAGCGTCACGGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K113845-0022-944-B03C-58kh
TTATAGCGTTTGAGAATTCTCGGCCGCCATAGCTTAAGACCTCTTTGAAGCTTGAAGACA
CACTTTCGTTTTCTACCTAATCTTCAAAAATTAGAAACAGAGTTTGCTTTTTTACCTGTA
GCCAAACACTATATTCATTATGGGCCTCAATTCGGCCTCACTTGGGCCTATATGTTATGA
AGAAATTGCAGAAACTTATATATATAGATTTTTTAATAAAATGTAAGAACAGGCCTGCCC
AAGATGGGCAAGAAATTGCAGAAATTGCCCAAGAAGTTTTTTTGTTCTTGAAATGAAATG
ATAAATTTGTAACATGAATCAGTAGTGATGGTCATGGTCTTGATCCATGTAGATTTCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGNTTTATTA
   
Sequence Name 51-K113844-0022-944-B03C-8409
GenBank Accession LN507568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K113844-0022-944-B03C-8409
TTGAATATATCCTGAAATCTACATGGATCAAGACCATGACCATCACTACTGATTCATGTT
ACAAATTTATCATTTCATTTCAAGAACAAAAAAACTTCTTGGGCAATTTCTGCAATTTCT
TGCCCATCTTGGGCAGGCCTGTTCTTACATTTTATTAAAAAATCTATATATATAAGTTTC
TGCAATTTCTTCATAACATATAGGCCCAAGTGAGGCCGAATTGAGGCCCATAATGAATAT
AGTGTTTGGCTACAGGTAAAAAAGCAAACTCTGTTTCTAATTTTTGAAGATTAGGTAGAA
AACGAAAGTGTGTCTTCAAGCTTCAAAGAGGTCTTAAGCTATGGCGGCCGAGAATTCTTC
AAACGCTATTAACGTCGATACGAGTCTCGATTCCGATTCAAAACCTAACCGTGACGCTAN
TTGATATG

Last Updated on 06.12.2018 16:11