Sequences from confirmation amplicon


Confirmation sequences for line 944E09 and hit At4g31590 are shown.
Line ID 944E09
Locus At4g31590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15311521
Primers used for PCR AY66 - 8474, show primer details
   
Sequence Name 41-K104738-0022-944-E09C-AY66
GenBank Accession LN504071 [GenBank]
Sequencing primer gene specific primer, AY66: 5'-TTCATCTTGTGTATGTTGGTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K104738-0022-944-E09C-AY66
TGCTCTCCATCAAGCTCTCTCCAGTTTTGTATTGTGCTGTTCCTTATACCGTTAGTAGAT
CGTTTGGTACTTTGCTTGGGTTGTTTTTGGATCAAGTATAAGAAAATTAAGCCGAGATTT
GATGAGGAACCTTTCCGTAATGATGATGCTGAGGGATCTGGATCTGAGTATCCAATGGTT
CTTGTTCAGATTCCAATGTGCAATGAGAGAGAGGTATGCTCATTCAACTTTGGTATATCC
AAGAAAGTATTCATCTTTCTCCTGCTTTATATAACCTTTTTGGTATCAGTGTAATGAGCT
TTAGTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTA
   
Sequence Name 42-K104739-0022-944-E09C-8409
GenBank Accession LN504455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104739-0022-944-E09C-8409
GCCATTTACATTGAAGACTAAAGCTCATTACACTGATACCAAAAAGGTCATATAAAGCAG
GAGAAAGATGAATACTTTCTTGGATATACCAAAGTTGAATGAGCATACCTCTCTCTCATT
GCACATTGGAATCTGAACAAGAACCATTGGATACTCAGATCCAGATCCCTCAGCATCATC
ATTACGGAAAGGTTCCTCATCAAATCTCGGCTTAATTTTCTTATACTTGATCCAAAAACA
ACCCAAGCAAAGTACCAAACGATCTACTGACTGTATAAGGAACAGCACAATACAAAACTT
GGAGAGAGCTTTGATGGGAGGAGCAATATAATCAGCTCTAAGGGTTAACCAACCAACATA
CACAAGATGA

Last Updated on 06.12.2018 16:11