Sequences from confirmation amplicon


Confirmation sequences for line 946F04 and hit At4g29160 are shown.
Line ID 946F04
Locus At4g29160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14381075
Primers used for PCR KU92 - 8474, show primer details
   
Sequence Name 39-K103307-0022-946-F04C-8409
GenBank Accession LN503320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103307-0022-946-F04C-8409
TTTAAAGTTTGGAGAGCATTAGCCTCCTGCTTGGGTTTCCCGAATAGCCGATTCATCATG
ATTTTGCTCCCACTTCGATCTCTTCTACCCCAACCGACACGATCACAGGTTACTGAAACA
ATTTTCCGCGAATTAAGAACCGATCGATCGATTAATCTACGAGGAAGATTCAGGATTCGT
AAGAATCGGATCAAAAAATACCTCTGTCCAAATCTGAATTGGAGATGGGGGAAGATCAAT
TGTATACGTAGACAAGAAATAATGGAAATCACTTGTATTGGTTTTTGTTTGTTATTTCGC
GTTTTTGTGTTCACAGAGAAGGGATTAAGAACGAAGGAAGCAACTTTCCTTTTTCCACAC
GCGTTGATGTGAATCGCGTTAACACGTGTTTATGTTTAAGTGGTTGTTTACAAAATAGTC
TCTTGCATGCGTTGTAGCCAATA
   
Sequence Name 15-K103308-0022-946-F04C-KU92
GenBank Accession LN492934 [GenBank]
Sequencing primer gene specific primer, KU92: 5'-AAACCAATATTATCGGCTACAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103308-0022-946-F04C-KU92
AATTTGTACACCACTTAACATAAACCGTGTTAACGCGATTCACATCAACGCGTGTGGAAA
AAGGAAAGTTGCTTCCTTCGTTCTTAATCCCTTCTCTGTGAACACAAAAACGCGAAATAA
CAAACAAAAACCAATACAAGTGATTTCCATTATTTCTCGTCTACGTATACAATTGATCTT
CCCCCATCTCCNATTCAGATTTGGACAGAGGTATTTTTTGATCCGATTCTTACGAATCCT
GAATCTTCCTCGTAGATTAATCGATCGATCGGTTCTTAATTCGCGGAAAATTGTTTCAGT
AACCTGTGATCGTGTCGGTTGGGGTAGAAGAGATCGAAGTGGGAGCAAAATCATGATGAA
TCGGCTATTCGGGAAACCCAAGCAGGAGGCTAATGCTCTCCAAACTTTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATATACGCACGGT

Last Updated on 06.12.2018 16:11