Sequences from confirmation amplicon


Confirmation sequences for line 947E12 and hit At4g20870 are shown.
Line ID 947E12
Locus At4g20870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11175183
Primers used for PCR EP79 - 8474, show primer details
   
Sequence Name 09-K103223-0022-947-E12C-8409
GenBank Accession LN490134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103223-0022-947-E12C-8409
TTGAATATATCCTGAAGAAAAAGGGTTTGAAATGAAACAGATAAGCTTCGTGATGAAATC
GGGTGTTTCTATGTTGCATAATCACAAATACAGAACTAACCAGTAACTCTTGGTTTGGAT
GTGGAAAAGGAAGCGATGAAGCGTGTACTCGAGCAGTGTCCACGTGAGTACACCAAAAGC
AACTATTAAACCGATCTGGGGAAACGTAAGTCCCTTACTCGCCGATATTGAAAGAACGTA
GCAAACAACTGGAAGCCAGATTGTTGGGATTGCCCACCAAACCGTGCGAGTCAAAAACTG
CTCAAATTTCCCAAAAAAAGGTTTAAGCCAAACATAGAGGCTTACATATATGAAGATAAA
TCTTAAAAAGGCAGAGTTGTTATGTTACCTCCCAAAAATCACTTTCAAAGGNGNNNNNGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAA
ATGGG
   
Sequence Name 57-K103230-0022-947-E12C-EP79
GenBank Accession LN509385 [GenBank]
Sequencing primer gene specific primer, EP79: 5'-GAAAGTGATTTTTGGGAGGTAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103230-0022-947-E12C-EP79
TGTAAGCCTCTATGTTTGGCTTAAACCTTTTTTTGGGAAATTTGAGCAGTTTTTGACTCG
CACGGTTTGGTGGGCAATCCCAACAATCTGGCTTCCAGTTGTTTGCTACGTTCTTTCAAT
ATCGGCGAGTAAGGGACTTACGTTTCCCCAGATCGGTTTAATAGTTGCTTTTGGTGTACT
CACGTGGACACTGCTCGAGTACACGCTTCATCGCTTCCTTTTCCACATCCAAACCAAGAG
TTACTGGTTAGTTCTGTATTTGTGATTATGCAACATAGAAACACCCGATTTCATCACGAA
GCTTATCTGTTTCATTTCAAACCCTTTTTTCTTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACGTGGATCAGCAATGAGTATGATGGTCAAAATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAA
ATGGG

Last Updated on 06.12.2018 16:11