Sequences from confirmation amplicon


Confirmation sequences for line 947F06 and hit At5g64700 are shown.
Line ID 947F06
Locus At5g64700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25866662
Primers used for PCR 46aq - 8474, show primer details
   
Sequence Name 08-K106755-0022-947-F06C-8409
GenBank Accession LN489748 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106755-0022-947-F06C-8409
GGAAGAGATGGAGCGAAAGCTTGACGATAGAAAACAAAGACGAAAGTGTTCATTCCACCG
TTGAAGACGGCTTTGGAGATCAAGAACATGATTGTATATATGACTTGTATTATACTAACC
ATCAGATATGGTTTCTTCGACTCCATGTCCATCTACCGATTTGATGATGTTTGGTTTTGG
TTTTGTTTTGAATTCACGGAGATGTATAAATTGTAGGGTTGTTCTTTCTCTCTCTGATAA
TCTAATGTTCTTCTTTTGAGTTAGAATAAGAAACTCTAAGTGGGTTATTTATATAAGTAC
AGACACAAGCACGCAAAAATTGGATATTTTACTACGGTGGTATTACAAAGTAATGGACTA
AATATTTTAAAAGTACGTAACTAAGAGTGGAGGATGAAAAAAAGACATTACTTTTGTGTC
ACAATCTCACTATACCAACCAAATTACATTATTTTTGATGTTGACCTCAAGAAAATTTCT
CGTTCCGTCCATTTATCCCCACGCCTACNAGATTTACTTTACTGTATGGTGTGATTTATA
GTTACATTTTGAACTCATAGATTTGAAGAAAATAAACATCATTTAACGAAAGTAACTTTC
GTAAAGAGAAACTGT
   
Sequence Name 24-K106756-0022-947-F06C-46aq
GenBank Accession LN497009 [GenBank]
Sequencing primer gene specific primer, 46aq: 5'-CACTGTGTCCACATGTTTCTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106756-0022-947-F06C-46aq
ATGTTATTTTCTTCAATCTATGAGTTCAAAATGTAACTATAAATCACACCATACAGTAAA
GTAAATCTAGTAGGCGTGGGGATAAATGGACGGAACGAGAAATTTTCTTGAGGTCAACAT
CAAAAATAATGTAATTTGGTTGGTATAGTGAGATTGTGACACAAAAGTAATGTCTTTTTT
TCATCCTCCACTCTTAGTTACGTACTTTTAAAATATTTAGTCCATTACTTTGTAATACCA
CCGTAGTAAAATATCCAATTTTTGCGTGCTTGTGTCTGTACTTATATAAATAACCCACTT
AGAGTTTCTTATTCTAACTCAAAAGAAGAACATTAGATTATCAGAGAGAGAAAGAACAAC
CCTACAATTTATACATCTCCGTGAATTCAAAACAAAACCAAAACCAAACATCATCAAATC
GGTAGATGGACATGGAGTCGAAGAAACCATATCTGATGGTTACTATAATACAAGTCATAT
ATACAATCATGTTCTTGATCTCCAAAGCCGTCTTCAACGGTGGAATGAACACTTTCGTCT
TTGTTTTCTATCGTCAAGCTTTCGCTACCATCTTCTTGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCC
Primers used for PCR 02ax - 8474, show primer details
   
Sequence Name 06-K106867-0022-947-F06C-8409
GenBank Accession LN488833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106867-0022-947-F06C-8409
CCGGAAGAGATGGTAGCGAAGCTTGACGATAGAAAACAAAGACGAAAGTGTTCATTCCAC
CGTTGAAGACGGCTTTGGAGATCAAGAACATGATTGTATATATGACTTGTATTATAGTAA
CCATCAGATATGGTTTCTTCGACTCCATGTCCATCTACCGATTTGATGATGTTTGGTTTT
GGTTTTGTTTTGAATTCACGGAGATGTATAAATTGTAGGGTTGTTCTTTCTCTCTCTGAT
AATCTAATGTTCTTCTTTTGAGTTAGAATAAGAAACTCTAAGTGGGTTATTTATATAAGT
ACAGACACAAGCACGCAAAAATTGGATATTTTACTACGGTGGTATTACAAAGTAATGGAC
TAAATATTTTAAAAGTACGTAACTAAGAGTGGAGGATGAAAAAAAGACATTACTTTTGTG
TCACAATCTCACTATACCAACCAAATTACATTATTTTTGATGTTGACCTCAAGAAAATTT
CTCGTTCCGTCCATTTATCCCCACGCCTACNAGATTTACTTTACTGTATGGTGTGATTTA
TAGTTACATTTTGAACTCATAGATTTGAAGAAAATAAACATCATTTAACGAAAGTAACTT
TCGTAAAGAGAAACATGTGGACACAGTGGTTTTGATGAAACAAAAT
   
Sequence Name 16-K106868-0022-947-F06C-02ax
GenBank Accession LN493449 [GenBank]
Sequencing primer gene specific primer, 02ax: 5'-TATTTTGTCCAATCAAAACCACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106868-0022-947-F06C-02ax
TTTCGTTAAATGATGTTTATTTTCTTCAAATCTATGAGTTCAAAATGTAACTATAAATCA
CACCATACAGTAAAGTAAATCTAGTAGGCGTGGGGATAAATGGACGGAACGAGAAATTTT
CTTGAGGTCAACATCAAAAATAATGTAATTTGGTTGGTATAGTGAGATTGTGACACAAAA
GTAATGTCTTTTTTTCATCCTCCACTCTTAGTTACGTACTTTTAAAATATTTAGTCCATT
ACTTTGTAATACCACCGTAGTAAAATATCCAATTTTTGCGTGCTTGTGTCTGTACTTATA
TAAATAACCCACTTAGAGTTTCTTATTCTAACTCAAAAGAAGAACATTAGATTATCAGAG
AGAGAAAGAACAACCCTACAATTTATACATCTCCGTGAATTCAAAACAAAACCAAAACCA
AACATCATCAAATCGGTAGATGGACATGGAGTCGAAGAAACCATATCTGATGGTTACTAT
AATACAAGTCATATATACAATCATGTTCTTGATCTCCAAAGCCGTCTTCAACGGTGGAAT
GAACACTTTCGTCTTTGTTTTCTATCGTCAAGCTTTCGCTACCATCTTCTTGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR 01ba - 8474, show primer details
   
Sequence Name 66-K118446-0022-947-F06C-01ba
GenBank Accession LN511706 [GenBank]
Sequencing primer gene specific primer, 01ba: 5'-GCTGTTCCTTGTATGCTCTTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118446-0022-947-F06C-01ba
CATAAGAATATTAGAACGAGTAATTAGGTATTAGTTAAAACTTGTCATACAAATATATAA
ACTTGTCATATTAGTAAGTTATACCCAAATAAAAGAGCCAAGAAGAATGTGATGGCCGGA
AGGGAAGCTGTTGTGGCGGCGGCTAAAGTTGCCGACGTGTATGATAATGCGATACCGTTT
AAGTCCAAGCTCAATGTCACACTGAAATCCCAATATATATAGATAAAGCTTTATTGATTA
TTATTAGTTCAACATAGATAAAATTTAACTGATTATAGTATACTAGTATAAGTATAGCAT
ACATACCCAAATAAAGAGAGCATGAAGATCTTGATGAAGGTCACAAATGAAAGAGGTGGA
GCACTTTTCCTAAAGAAAAAAGGAGAGAATACAATTAACATAAATAAAAATTAACGTTTA
CAATATTAATATATTTGCATATATATATTAATATTAACGTTTGCATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 26-K118445-0022-947-F06C-8409
GenBank Accession LN498133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118445-0022-947-F06C-8409
ACATTGAATATATGCAAACGTTAATATTAATATATATATGCAAATATATTAATATTGTAA
ACGTTAATTTTTATTTATGTTAATTGTATTCTCTCCTTTTTTCTTTAGGAAAAGTGCTCC
ACCTCTTTCATTTGTGACCTTCATCAAGATCTTCATGCTCTCTTTATTTGGGTATGTATG
CTATACTTATACTAGTATACTATAATCAGTTAAATTTTATCTATGTTGAACTAATAATAA
TCAATAAAGCTTTATCTATATATATTGGGATTTCAGTGTGACATTGAGCTTGGACTTAAA
CGGTATCGCATTATCATACACGTCGGCAACTTTAGCCGCCGCCACAACAGCTTCCCTTCC
GGCCATCACATTCTTCTTGGCTCTTTTATTTGGGTATAACTTACTAATATGACAAGTTTA
TATATTTGTATGACAAGTTTTAACTAATACCTAATTACTCGTTCTAATATTTCTTTATTG
TTTATTAAATCAAAGAATGGAGAGGCTTAAGGTTAAGAGCATACAGGGAACAGC

Last Updated on 06.12.2018 16:11