Sequences from confirmation amplicon


Confirmation sequences for line 948C05 and hit At3g56640 are shown.
Line ID 948C05
Locus At3g56640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20984197
Primers used for PCR 90ra - 8474, show primer details
   
Sequence Name 76-K116549-0022-948-C05C-90ra
GenBank Accession LN513454 [GenBank]
Sequencing primer gene specific primer, 90ra: 5'-AGCCTATCTTGCTTTGTTGAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K116549-0022-948-C05C-90ra
AATGTTACTGGACTACAGAGGAAATGCCACAAGGGCCGCACGAGTACATAAACGAGGTTG
TTATATACCTCGAGACTGTTATGTCAACGGCACAACAGATCCTCCCAATGGATGCCTTAT
ACAAAGTTGGAGTTGGGGCTATCGAACATATCTCAAACTCCATCGTCTCTACATTCCTCA
GCGATAGCATCAAGAGATTCAATGCTAATGCAGTCTCGGCCATTAACCACGATCTGAGAG
TGATAGAAAACTTTGCTGATGAGAGATATCACTCAAGCGGGTTAAATGAGATCTACAAAG
AAGGAAGCTTTAGAAGCTATCTGGTTGAAGCTAGGCAGTTGATAAACCTGTTGTCGAGTA
GCCAACCCGAGAACTTCATGAACCCGGTGATTAGAGAGAGGAACTACAACACTTTGGATT
ACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CC
   
Sequence Name 36-K116548-0022-948-C05C-8409
GenBank Accession LN502367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116548-0022-948-C05C-8409
TACATTGTAATCCAAAGTGTTGTAGTTCCTCTCTCTAATCACCGGGTTCATGAAGTTCTC
GGGTTGGCTACTCGACAACAGGTTTATCAACTGCCTAGCTTCAACCAGATAGCTTCTAAA
GCTTCCTTCTTTGTAGATCTCATTTAACCCGCTTGAGTGATATCTCTCATCAGCAAAGTT
TTCTATCACTCTCAGATCGTGGTTAATGGCCGAGACTGCATTAGCATTGAATCTCTTGAT
GCTATCGCTGAGGAATGTAGAGACGATGGAGTTTGAGATATGTTCGATAGCCCCAACTCC
AACTTTGTATAAGGCATCCATTGGGAGGATCTGTTGTGCCGTTGACATAACAGTCTCGAG
GTATATAACAACCTCGTTTATGTACTCGTGCGGCCCTTGTGGCATTTCCTCTGTAGTCCA
GTTAACATTTTCTGTGAGCTTCATGAACTCGTCCAACTTGGTGTTAACCACATTCAACA

Last Updated on 06.12.2018 16:11