Sequences from confirmation amplicon


Confirmation sequences for line 948C07 and hit At4g08300 are shown.
Line ID 948C07
Locus At4g08300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5247054
Primers used for PCR 32ty - 8474, show primer details
   
Sequence Name 36-K117118-0022-948-C07C-32ty
GenBank Accession LN502379 [GenBank]
Sequencing primer gene specific primer, 32ty: 5'-CGGTTATGGGTAGTATCACCACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117118-0022-948-C07C-32ty
TTCCAATTGGTGTGTTTACATTAATTAGTAAATGTGTTATACCATACTATTATAGTTATT
CTGATCACTATAACATAACAAAGAGTGGTACCAAATCTCCATTTTGACTAGGCCTAGGCA
AAATAGCCAAACCCGAAGAACCAAACCGAACCCGACCCGAAAAACCCGATCTGAATTCGA
ACCCAAAACTTTAAAATACCCGAACGGATCCTAAACTTCAAAACCCGAACCCGATCCGAA
CCTAAATACTCGATCAAGTACCCGAACATATCCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 14-K117117-0022-948-C07C-8409
GenBank Accession LN492684 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117117-0022-948-C07C-8409
ATTTACATTGGATATGTTCGGGTACTTGATCGAGTATTTAGGTTCGGATCGGGTTCGGGT
TTTGAAGTTTAGGATCCGTTCGGGTATTTTAAAGTTTTGGGTTCGAATTCAGATCGGGTT
TTTCGGGTCGGGTTCGGTTTGGTTCTTCGGGTTTGGCTATTTTGCCTAGGCCTAGTCAAA
ATGGAGATTTGGTACCACTCTTTGTTATGTTATAGTGATCAGAATAACTATAATAGTATG
GTATAACACATTTACTAATTAATGTAAACACACCAATTTGGAATATAAGTAACTAATGTT
TGCTTACTTGTAGAATGAAGAAACCTGCCCAAGTGGTGATACTACCCATAACCG

Last Updated on 06.12.2018 16:11