Sequences from confirmation amplicon


Confirmation sequences for line 948F06 and hit At1g69730 are shown.
Line ID 948F06
Locus At1g69730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26230124
Primers used for PCR 44lx - 8474, show primer details
   
Sequence Name 21-K114086-0022-948-F06C-44lx
GenBank Accession LN495753 [GenBank]
Sequencing primer gene specific primer, 44lx: 5'-TTGATCTGTGAAAGAATGACAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114086-0022-948-F06C-44lx
GACTTTGGACTTTTTACTGCAACGATTCTTCCATCGACAAGCATTCCCTTGTACACTGTA
CCTTGACCACCCTGGCCAAGTATTCTGTTTGAACTGAAGTTTTCAGTAGCTTTCTCCAAC
TCTCGCGAACTGAACACTATTGTCTTTTCGACCATGCCTACTGTTGAAATCAATTGTTGT
TGCAACAATAGACCACCATTACGCTTGAAGAACTTCTTCTTTTGGTTTAACTTCCTTTGC
TTTTTAATAAATTTGTATAACAAGTATATTCCAACAACAAAGATCAATGAGCCAAAACTA
GCACCAAGCCCTGCAAACACAGACTTGATCATCATTGTTGAATATTAAAAAAATAACTAA
TGTTGGGCATCACCATTGTAAAAGAGCTTTAAATGGACATGCGCGTGTATTTGGATTTTT
GGCATAAAAATTCCCACGACTTACCTATAGCAAGTGGCCGATGATTGTTGTACACACATT
TAAAGTGTCCTTGCAAATTGGTCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 45-K114085-0022-948-F06C-8409
GenBank Accession LN505606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114085-0022-948-F06C-8409
TTTATGTGTGTACAACAATCATCGGCCACTTGCTATAGGTAAGTCGTGGGAATTTTTATG
CCAAAAATCCAAATACACGCGCATGTCCATTTAAAGCTCTTTTACAATGGTGATGCCCAA
CATTAGTTATTTTTTTAATATTCAACAATGATGATCAAGTCTGTGTTTGCAGGGCTTGGT
GCTAGTTTTGGCTCATTGATCTTTGTTGTTGGAATATACTTGTTATACAAATTTATTAAA
AAGCAAAGGAAGTTAAACCAAAAGAAGAAGTTCTTCAAGCGTAATGGTGGTCTATTGTTG
CAACAACAATTGATTTCAACAGTAGGCATGGTCGAAAAGACAATAGTGTTCAGTTCGCGA
GAGTTGGAGAAAGCTACTGAAAACTTCAGTTCAAACAGAATACTTGGCCAGGGTGGTCAA
GGTACAGTGTACAAGGGAATGCTTGTCGATGGAAGAATCGTTGCAGTAAAAAAGTCCAAA
GTCGTGGACGAAGACAAGCTAGAAGAGTTCATCAATGAGGTTGTCATTCTTTCACAGATC
A

Last Updated on 06.12.2018 16:11