Sequences from confirmation amplicon


Confirmation sequences for line 949G04 and hit At3g47740 are shown.
Line ID 949G04
Locus At3g47740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17603787
Primers used for PCR 84ns - 8474, show primer details
   
Sequence Name 73-K114716-0022-949-G04C-84ns
GenBank Accession LN512952 [GenBank]
Sequencing primer gene specific primer, 84ns: 5'-AAGAAAGGTTTATCCTGGTAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114716-0022-949-G04C-84ns
CCCTCGCTGTGCCTTCGGAGAATGCTTTGGCATGTTAGGGCCCAATGGTGCTGGCAAGAC
CTCTTTTATCAATATGGTCAGTACACAAATCTACCCCTAAAAAATAAATAGTTGTGCTTA
GCTTATGGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 25-K114715-0022-949-G04C-8409
GenBank Accession LN497565 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114715-0022-949-G04C-8409
ATTTACAATTGAAATATCCTGACCATAAGCTAAGCACAACTATTTATTTTTTAGGGGTAG
ATTTGTGTACTGACCATATTGATAAAAGAGGTCTTGCCAGCACCATTGGGCCCTAACATG
CCAAAGCATTCTCCTGAAGGCACAGCGAGGGATAACACTCGAACTGCCTTTTTAGGCGGG
TTCCCATCCCTACCAGGATAAACCTTTCT

Last Updated on 06.12.2018 16:11