Sequences from confirmation amplicon


Confirmation sequences for line 951B07 and hit At1g23550 are shown.
Line ID 951B07
Locus At1g23550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8352183
Primers used for PCR 21kz - 8474, show primer details
   
Sequence Name 72-K113934-0022-951-B07C-21kz
GenBank Accession LN512765 [GenBank]
Sequencing primer gene specific primer, 21kz: 5'-GTGCTAAAGGATTGGTTGGTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113934-0022-951-B07C-21kz
AGCTCGCCGTGGGTTAGTTTTGCTTCCTTGATGTCAATGTTGTCTACATCAATGGATCCT
TCAAGAATGAACTTGATCATCCGAACTTACGATGATTTTCGGGTATGTAATCCAGTTTCC
TTACTGTATGTTCTGTTCTATTATTCTTGAAGTTCGTTGGTTAAGATTCAATGTGGGTTT
ATTTTGCAGAAACGGAAGATTAGGAGAGACCAATTGGTAAGGAAGATGAGAGAAGTGGCT
GGAGATAATCTTTTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 37-K113933-0022-951-B07C-8409
GenBank Accession LN502691 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K113933-0022-951-B07C-8409
TTGAATATATAAAAGATTATCTCCAGCCACTTCTCTCATCTTCCTTACCAATTGGTCTCT
CCTAATCTTCCGTTTCTGCAAAATAAACCCACATTGAATCTTAACCAACGAACTTCAAGA
ATAATAGAACAGAACATACAGTAAGGAAACTGGATTACATACCCGAAAATCATCGTAAGT
TCGGATGATCAAGTTCATTCTTGAAGGATCCATTGATGTAGACAACATTGACATCAAGGA
AGCAAAACTAACCCACGGCGAGCTTGGTCTCGAAGCAAAACCACCTCTACTCACTGAAAA
AAATACACCAACCAATCCTTTAGCACA

Last Updated on 06.12.2018 16:11