Sequences from confirmation amplicon


Confirmation sequences for line 953A01 and hit At5g19290 are shown.
Line ID 953A01
Locus At5g19290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6495111
Primers used for PCR 47lx - 8474, show primer details
   
Sequence Name 46-K114144-0022-953-A01C-47lx
GenBank Accession LN505932 [GenBank]
Sequencing primer gene specific primer, 47lx: 5'-TTTATGATTTGAGCTCTTGTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114144-0022-953-A01C-47lx
GTTGACCATTATTTGAAATTTTCTGAAATTGGTAGAGAATCTGAAACATTAATTTTCAAT
ATTTTTTTCTCTTCTTGTCAACGATTTTTTATATTATTGATGATACCCAAAGGTATTGGT
TTAACTGTAAACCATGTGCTACGATTACGAAACAGTAAAACCATAAATAGTTTCTTGCCA
GATGGCACAGTCCTATTGGACTACAATACAACACCCAAAGACTCAAAAGAAAGAAAGAGT
AAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
G
   
Sequence Name 19-K114143-0022-953-A01C-8409
GenBank Accession LN494851 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114143-0022-953-A01C-8409
CATTTACTTTACTCTTTCTTTCTTTTGAGTCTTTGGGTGTTGTATTGTAGTCCAATAGGA
CTGTGCCATCTGGCAAGAAACTATTTATGGTTTTACTGTTTCGTAATCGTAGCACATGGT
TTACAGTTAAACCAATACCTTTGGGTATCATCAATAATATAAAAAATCGTTGACAAGAAG
AGAAAAAAATATTTGAAAATTAATGTTTCAGATTCTCTACCAATTTCAGAAAATTTTCAA
ATAATGGTCAACCATCTGATAATGTCGTTGTTTTCTCCGTCAAGGGACAAGAGCTCANAT
CATAA

Last Updated on 06.12.2018 16:11