Sequences from confirmation amplicon


Confirmation sequences for line 954D03 and hit At5g56720 are shown.
Line ID 954D03
Locus At5g56720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22946447
Primers used for PCR 25du - 8474, show primer details
   
Sequence Name 58-K110309-0022-954-D03C-25du
GenBank Accession LN509732 [GenBank]
Sequencing primer gene specific primer, 25du: 5'-CTATCTTTAGTTCACGATCCGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K110309-0022-954-D03C-25du
CTGTTCTTTGTCTTGTCTCCGTAATGTAACATTGCAATCACTAAGATATGAATTTGGATC
TTTGGTTACTAGATTTTGGCTGGCATTGCGGGATTGGGTGTTGTTGTTTTTGGCATCTGG
AGATACTCTCGCAAGTAGAAGTATGAACTCTCTTTCTTTCAATTTTCTGATTATTTAACC
CCAAGATTTCAGAGTTTTTAAATGTATCTGTGAACCTCAGCGTTTATTATGTAAGTTCCC
ATTATTTTTCATGAATATAGAAACACAAACTTATTTGTTTGCTACATTGTTTGTGCCATG
ACTATCATAGTTTTCAACTTTTGGTCTATACATTGAGACAGGAATAAGCTAAATCCTTCT
CTTCAGCTAGCTCTCGTGCAGAGTCATCCATTTTCTCCCTCGAAAACTCGTCTATACTGA
GTCCTTGTACGATCTTCCAACTCCCTTTCTCACAGATAACCGGAAACGAGTAAACCAAAC
CAGGTGGTATACCATATGAGCCGTCAGAACAAACACCCATAGAAACCCAAGTTCCTTTAG
GGGTTCCAAGAAACCAATCACGGATATGGTCACAAGCTGCACCAGCAGCGGAAAAAGCAC
TCGACTGTAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 45-K110308-0022-954-D03C-8409
GenBank Accession LN505560 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K110308-0022-954-D03C-8409
CTTTTTCCGCTGCTGGTGCAGCTTGTGACCATATCCGTGATTGGTTTCTTGGAACCCCTA
AAGGAACTTGGGTTTCTATGGGTGTTTGTTCTGACGGCTCATATGGTATACCACCTGGTT
TGGTTTACTCGTTTCCGGTTATCTGTGAGAAAGGGAGTTGGAAGATCGTACAAGGACTCA
GTATAGACGAGTTTTCGAGGGAGAAAATGGATGACTCTGCACGAGAGCTAGCTGAAGAGA
AGGATTTAGCTTATTCCTGTCTCAATGTATAGACCAAAAGTTGAAAACTATGATAGTCAT
GGCACAAACAATGTAGCAAACAAATAAGTTTGTGTTTCTATATTCATGAAAAATAATGGG
AACTTACATAATAAACGCTGAGGTTCACAGATACATTTAAAAACTCTGAAATCTTGGGGT
TAAATAATCAGAAAATTGAAAGAAAGAGAGTTCATACTTCTACTTGCGAGAGTATCTCCA
GATGCCAAAAACAACAACACCCAATCCCGCAATGCCAGCCAAAATCTAGTAACCAAAGAT
CCAAATTCATATCTTAGTGATTGCAATGTTACATTTACGGAGACAAGACAAAGAACAGAT
GAACACTGACCTTGGTATCACGGATATAAGGGCCCTCGGATCGTGA

Last Updated on 06.12.2018 16:11