Sequences from confirmation amplicon


Confirmation sequences for line 954F12 and hit At5g58910 are shown.
Line ID 954F12
Locus At5g58910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23790565
Primers used for PCR KF08 - 8474, show primer details
   
Sequence Name 17-K103103-0022-954-F12C-KF08
GenBank Accession LN493808 [GenBank]
Sequencing primer gene specific primer, KF08: 5'-CTGTAAAGCTTTGTCCCTTTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103103-0022-954-F12C-KF08
CGGTATAGTCATACGGATTGGAAGGTTTCGCCGGGAAGTCGTCGGTAAATACGCCGGAAA
TGTTAAAGAAATGAGCCTGGAGTAAAGCGGTTTTAGGCATGGTAAACGTTACGTTGTTTA
TTCCGGCCACTAAGCGAACACCGTTGTTGCAACTCTGGCAAGGGTTTGCACCAAGACCTA
CCGTGAAGAACAAGGAATGTTCCACGGTTGTTGGAACCCTAGCTGGATACTCGAGGGAGT
TTAAGCTCCTGAGTGATCTAGTGAATTTGGTTGCAACCCATGTTGCGTTTTGAGGTGGAA
GTGATGCGAGAACGGTTTTTTTGGAGGTGGAAACGGTGGAAGTGTGACCGATATAGTGGA
GGGTGGCAGTGGCTGTGACGTTGTCGTATGGGATGTGAGCGTCCGTAAATGGCTATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACAGATTATT
   
Sequence Name 17-K103104-0022-954-F12C-8409
GenBank Accession LN493809 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103104-0022-954-F12C-8409
GCCATTTACAATTGAATATAGCCATTTACGGACGCTCACATCCCATACGACATCGTCACA
GCCACTGCCACCCTCCACTATATCGGTCACACTTCCACCGTTTCCACCTCCAAAAAAACC
GTTCTCGCATCACTTCCACCTCAAAACGCAACATGGGTTGCAACCAAATTCACTAGATCA
CTCAGGAGCTTAAACTCCCTCGAGTATCCAGCTAGGGTTCCAACAACCGTGGAACATTCC
TTGTTCTTCACGGTAGGTCTTGGTGCAAACCCTTGCCAGAGTTGCAACAACGGTGTTCGC
TTAGTGGCCGGAATAAACAACGTAACGTTTACCATGCCTAAAACCGCTTTACTCCAGGCT
CATTTCTTTAACATTTCCGGCGTATTTACCGACGACTTCCCGGCGAAACCTTCCAATCCG
TATGACTATACCGCACCAGTAAAGCTCGGCGTTAATGCTGCGACGATGAAAGGGACAAAG
CTTTACA

Last Updated on 06.12.2018 16:11