Sequences from confirmation amplicon


Confirmation sequences for line 954H06 and hit At3g62150 are shown.
Line ID 954H06
Locus At3g62150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23009445
Primers used for PCR AC89 - 8474, show primer details
   
Sequence Name 12-K104226-0022-954-H06C-AC89
GenBank Accession LN491556 [GenBank]
Sequencing primer gene specific primer, AC89: 5'-GAAACAGGTTTTCTTTGCTTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104226-0022-954-H06C-AC89
CAGTTCTTGTCTCCTGATTCCGCAAAGCTAGCAATGCGGCTGCATCGATCTTCGCGGTTA
TAGACAGAGAATCGAAGATTGATCCAAGTGATGAGTCTGGGAGAGTTCTTGATAATGTTA
AAGGAGATATCGAACTTCGTCATATTAGTTTCAAATATCCTTCAAGACCAGATGTTCAGA
TCTTCCAAGATCTCTGTCTGTCTATTCGCGCTGGCAAGGTAAGATATAGAAAAAATGAAT
CAGTTTGGTTCAGTTCGGGTCAAATAAAGTCTCTTATTTTGTTTTGTTTTTCCCTCTGTT
TCAGACAATAGCTTTGGTTGGAGAGAGCGGGAGCGGGAAATCGACAGTGATTGCGTTGCT
ACAGAGGTTTTACGACCCTGATTCTGGTCAGATCACTCTTGATGGAGTTGAGATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
A
   
Sequence Name 28-K104227-0022-954-H06C-8409
GenBank Accession LN498823 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104227-0022-954-H06C-8409
CGGACTGAGCCATTTACATTGAATATCTCAACTCCATCAAGAGTGATCTGACCAGAATCA
GGGTCGTAAAACCTCTGTAGCAACGCAATCACTGTCGATTTCCCGCTCCCGCTCTCTCCA
ACCAAAGCTATTGTCTGAAACAGAGGGAAAAACAAAACAAAATAAGAGACTTTATTTGAC
CCGAACTGAACCAAACTGATTCATTTTTTCTATATCTTACCTTGCCAGCGCGAATAGACA
GACAGAGATCTTGGAAGATCTGAACATCTGGTCTTGAAGGATATTTGAAACTAATATGAC
GAAGTTCGATATCTCCTTTAACATTATCAAGAACTCTCCCAGACTCATCACTTGGATCAA
TCTTCGATTCTCTGTCTATAACCGCGAAGATCGATGCAGCCGCATTGCTAGCTTTGCTGG
AATCAGGAGACAATGAACTTGACTGAGAAATAGCTACAGCCGCCATTGTCAAAGCAAAGA
AAACCTGTTT

Last Updated on 06.12.2018 16:11