Sequences from confirmation amplicon


Confirmation sequences for line 955B11 and hit At5g36230 are shown.
Line ID 955B11
Locus At5g36230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14274969
Primers used for PCR 15lm - 8474, show primer details
   
Sequence Name 25-K114086-0022-955-B11C-15lm
GenBank Accession LN497543 [GenBank]
Sequencing primer gene specific primer, 15lm: 5'-CTGTGATTTCTGATGTCCACTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114086-0022-955-B11C-15lm
TGTTGACTGTATATCTATTTACCTGTATATTCTGCAGTGTAAATGGTTCTGTAGAAATAT
TCCTGGATATTTTTCTTCTTCTTGTTATTGTGGTTCTGTTTTCTTCTATTCAAGGGGTGT
TTGTGTTATAATTTCTTATGCCTTCTACTGGTTTTGTTTGGTTTGCCCTTTTTAGTTATG
AATTTTGATTGAGAGATCTTGTGTTTTAAGGTTATTTTCATAGGAGGACGCACACAACCT
GGAACAGTGAAATCTGATGAAGGAGAACGTCATACTTACTCTGTAATTGATTGTGAACCC
ATAAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA
   
Sequence Name 49-K114085-0022-955-B11C-8409
GenBank Accession LN506916 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K114085-0022-955-B11C-8409
CATTTACAATTGAATTATGGGTTCACATCAATTACAGAGTAAGTATGACGTTCTCCTTCA
TCAGATTTCACTGTTCCAGGTTGTGTGCGTCCTCCTATGAAAATAACCTTAAAACACAAG
ATCTCTCAATCAAAATTCATAACTAAAAAGGGCAAACCAAACAAAACCAGTAGAAGGCAT
AAGAAATTATAACACAAACACCCCTTGAATAGAAGAAAACAGAACCACAATAACAAGAAG
AAGAAAAATATCCAGGAATATTTCTACAGAACCATTTACACTGCAGAATATACAGGTAAA
TAGATATTACAGGTTCAACAGATTGAACACGTATTCTTCTATACAAGTGGACATCAANAA
TCACA

Last Updated on 06.12.2018 16:11