Sequences from confirmation amplicon


Confirmation sequences for line 956B10 and hit At2g37620 are shown.
Line ID 956B10
Locus At2g37620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15780664
Primers used for PCR 23ie - 8474, show primer details
   
Sequence Name 48-K112810-0022-956-B10C-23ie
GenBank Accession LN506546 [GenBank]
Sequencing primer gene specific primer, 23ie: 5'-GTACCCTATTGAGCATGGAATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112810-0022-956-B10C-23ie
CATCCACTTTCTACAATGAGCTCCGTGTTGCTCCTGAGGAACATCCTATTCTACTTACCG
AGGCACCGCTTAACCCGAAAGCTAATCGTGAGAAGATGACTCAAATCATGTTTGAGACTT
TCAATGCCCCTGCTATGTATGTGGCTATTCAGGCTGTTCTTTCTCTTTATGCCAGTGGTC
GTACTACCGGTGAGTGTAGCGCTTTTGTGTCCTTATGGTGTAAAGCTTCGATCTTTGTAC
ATTTTTGCAGAGCAAAACGTAAAGCCTTTCTTGAATTGTGGATGTGCAGGTATTGTGCTC
GACTCTGGAGATGGTGTGAGCCACACTGTTCCTATCTATGAGGGGTATGCACTTCCACAT
GCTATCCTACGTCTTGATCTTGCTGGTCGTGACCTCACGGATGCGCTGATGAAGATCCTA
ACCGAGCGTGGTTACTCTTTCACCACCACAGCAGAGCGTGAAATTGTCAGAGACATAAAG
GAGAAGCTTTGCTACATTGCTCTTGACTACGAGCAGGAACTACGAGAAATCTACATGGAT
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 23-K112809-0022-956-B10C-8409
GenBank Accession LN496627 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K112809-0022-956-B10C-8409
TCAGAGCAATGTAGCAAAGCTTCTCCTTTATGTCTCTGACCCTTTAACGCTCTGCTGTGG
TGGTGAAAGAGTAACCACGCTCGGCTATGATCTTCATCAGCGCATCCGTGAGGTCACGAC
CAGCAAGATCAAGACGTAGGATAGCATGTGGAAGTGCATACCCCTCATAGATAGGAACAG
TGTGGCTCACACCATCTCCAGAGTCGAGCACAATACCTGCACATCCACAATTCAAGAAAG
GCTTTACGTTTTGCTCTGCAAAAATGTACAAAGATCGAAGCTTTACACCATAAGGACACA
AAAGCGCTACACTCACCGGTAGTACGACCACTGGCATAAAGAGAAAGAACAGCCTGAATA
GCCACATACATAGCAGGGGCATTGAAAGTCTCAAACATGATTTGAGTCATCTTCTCACGA
TTAGCTTTCGGGTTAAGCGGTGCCTCGGTAAGTAGAATAGGATGTTCCTCAGGAGCAACA
CGGAGCTCATTGTAGAAAGTGTGATGCCAAATCTTCTCCATGTCATCCCAATTGTTGACA
ATTCCATGCTCANTAGGGTA

Last Updated on 06.12.2018 16:11