Sequences from confirmation amplicon


Confirmation sequences for line 956B12 and hit At4g30440 are shown.
Line ID 956B12
Locus At4g30440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14882319
Primers used for PCR 32xs - 8474, show primer details
   
Sequence Name 57-K119546-0022-956-B12C-32xs
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 32xs: 5'-TACTTCACAGCTGGACCGTAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K119546-0022-956-B12C-32xs
ATCCACGGTTACCGTACCCACCACTTTCTCTGCTGTCCCACTCTTCCCAGCTGTCCTTAT
TCCCTTTTTTTTATTTTATTTCTTTTTCTAAAAGATCCCTAACAAATTATTTTGGTAGGA
AAATATTTGCTGCCTTACATCAATCAATTCTGTATTAAGAAAACAAAAATGTTCAAATAC
ATTTCAAGATTAACACAATCACGATTAACTTTAAATTAACGCGGTATAATAGGCTGTCCT
TTTTTCGGTTTTTGCTTGTCTTAAGAGAAACGAATCATTTAGATTTTATAATAATCCTAA
AAGAAACGAATCATATAAAATGTATGGGAGAAAGAAAAAAATAATTTCATTAAAAATAAT
AATAATAGTCACGGTTAATGTACAAGCTTGGCTTTAGTATTGTATCCGTAATAAGAAAGA
TACCATCTAACGAACTTCTTCAACCCGGTTTCCAAATCGGTTGTCGGTTTATACCCGAAT
TCATTTCGGGCTGAGCTAATATTGGCATGTGTGAACGGTACGTCGCCGTTTCCTGGCATC
TCCACGAAGTTCCTCTTCGCTTTCACCTTTAAATGCTTCTCCAATATATCCACCAGAATC
GGTACTGTAACCGGAGATGTGTTTCCCAGGTTAAATATCCGGTACGGTGCTGCTCCACGT
TTTTTACCACCCGACCCGGTACTTTTACCCGATGAATCCAGAGATCCTAAACATCCTTTC
AGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
   
Sequence Name 25-K119545-0022-956-B12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119545-0022-956-B12C-8409
TGAAGGATGTTTAGGATCTCTGGATTCATCGGGTAAAAGTACCGGGTCGGGTGGTAAAAA
ACGTGGAGCAGCACCGTACCGGATATTTAACCTGGGAAACACATCTCCGGTTACAGTACC
GATTCTGGTGGATATATTGGAGAAGCATTTAAAGGTGAAAGCGAAGAGGAACTTCGTGGA
GATGCCAGGAAACGGCGACGTACCGTTCACACATGCCAATATTAGCTCAGCCCGAAATGA
ATTCGGGTATAAACCGACAACCGATTTGGAAACCGGGTTGAAGAAGTTCGTTAGATGGTA
TCTTTCTTATTACGGATACAATACTAAAGCCAAGCTTGTACATTAACCGTGACTATTATT
ATTATTTTTAATGAAATTATTTTTTTCTTTCTCCCATACATTTTATATGATTCGTTTCTT
TTAGGATTATTATAAAATCTAAATGATTCGTTTCTCTTAAGACAAGCAAAAACCGAAAAA
AGGACAGCCTATTATACCGCGTTAATTTAAAGTTAATCGTGATTGTGTTAATCTTGAAAT
GTATTTGAACATTTTTGTTTTCTTAATACAGAATTGATTGATGTAAGGCAGCAAATATTT
TCCTACCAAAATAATTTGTTAGGGATCTTTTAGAAAAAGAAATAAAATAAAAAAAAGGGA
ATAAGGACAGCTGGGAAGAGTGGGACAGCAGAGAAAGTGGTGGTGTACGGTAACCGTTGG
ATCCGATGAGGAATTTAATAAAGGTAGTCGTAATCTTACGGTCC

Last Updated on 06.12.2018 16:11