Sequences from confirmation amplicon


Confirmation sequences for line 958D07 and hit At2g34810 are shown.
Line ID 958D07
Locus At2g34810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14686797
Primers used for PCR 24my - 8474, show primer details
   
Sequence Name 36-K114505-0022-958-D07C-24my
GenBank Accession LN502322 [GenBank]
Sequencing primer gene specific primer, 24my: 5'-TTAAAAAGACGCGTAAAATCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114505-0022-958-D07C-24my
AATATTTAGATTTTGCATCAGTATTCTTTGCAATTAAAGACCATATATTGTTCATTTATG
CTATAACTAAAACTAATCATATATTGTTGATTATAATCAATGAATTCTCTTAGAGATATA
CAACTCCGATCCATTTATTATTAAAAATTTTAGCACATAACTTCTCTTGGAGATTGAAGT
AAACCATTATTTAACACAAAATTATCAACTGCCCATGCCAGAACAACTAAGGAACATATA
TACCCAATAGATTCATTGGATATGATCCCAAATTTCAACTTTTAATAACTTAATAGTATA
CTCCTTCCCACACAACAATACAATCAATTCAAACACTCACCAATCACCAAACTAAGCAAG
AACCGGAATCCCTTGCTGAGACCGGAAAAAATTAGTCCGGTCGAACCGGGCTTTAATCTT
AACCAATCTCAAATAATTCCCTTTGAAATAACTATCCCCATAAACCTTAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 11-K114504-0022-958-D07C-8409
GenBank Accession LN491218 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114504-0022-958-D07C-8409
ATTTCATTAAGGTTTATGGGGATAGTTATTTCAAGGGAATTATTTGAGATTGGTTAAGAT
TAAAGCCCGGTTCGACCGGACTAATTTTTTCCGGTCTCAGCAAGGGATTCCGGTTCTTGC
TTAGTTTGGTGATTGGTGAGTGTTTGAATTGATTGTATTGTTGTGTGGGAAGGAGTATAC
TATTAANTTATTAAAAGTTNAAATTTGGGATNATATANAATGAATNTATTGGGTATATAT
GTTGGTTAGTTGTTNTGGNATGGGNAGTTGATAATTTTGTGTTAAATAATGGTTTANTTG
AATTTNAAAGAGAAGTTATGTGNTAAAATTTTTAATAATAAATGGATNGGAGTTGTATAT
NTNTAAGAGAATTNATTGATTATAATGAANAATATATGATTAGTTTTAGTTATAGNATAA
ATGAANAATATATGGTNTTTAATTTGNAAAGAATANTGATTGNAAAATTTTAAATAAATT
TNATAATAAAAAATNNAAAAGATTTTATGGGTATTTTTA

Last Updated on 06.12.2018 16:11